Isolation of a DNA sequence stimulating recombination in yeast.
Acta Microbiol Hung
; 36(1): 61-5, 1989.
Article
em En
| MEDLINE
| ID: mdl-2690556
ABSTRACT
A series of DNA sequences was rescued from the yeast Saccharomyces cerevisiae transformed by a gene library and selected for the cdc35ts+, TRP1+ phenotype. These sequences did not complement the cdc35ts mutation, and were found in various amounts and orientations in degraded plasmids. A similar phenomenon was demonstrated when the HIS3 gene was cloned into one of them a highly deleted plasmid was rescued from complemented homozygous diploid yeast cells, in which the HIS3+/his3- character was inherited at a 22 ratio. These results suggest that the insert sequences rescued from the cdc35ts transformants stimulate vigorous non-reciprocal recombination events by the transfer of HIS3 gene or the TRP-ARS fragment. This event was detected in the transformation of cdc35 or his3- hosts and was followed by the re-isolation of the degraded plasmid molecules.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Plasmídeos
/
Recombinação Genética
/
Saccharomyces cerevisiae
Idioma:
En
Revista:
Acta Microbiol Hung
Ano de publicação:
1989
Tipo de documento:
Article
País de afiliação:
Hungria