Unusual RNA plant virus integration in the soybean genome leads to the production of small RNAs.
Plant Sci
; 246: 62-69, 2016 May.
Article
em En
| MEDLINE
| ID: mdl-26993236
ABSTRACT
Horizontal gene transfer (HGT) is known to be a major force in genome evolution. The acquisition of genes from viruses by eukaryotic genomes is a well-studied example of HGT, including rare cases of non-retroviral RNA virus integration. The present study describes the integration of cucumber mosaic virus RNA-1 into soybean genome. After an initial metatranscriptomic analysis of small RNAs derived from soybean, the de novo assembly resulted a 3029-nt contig homologous to RNA-1. The integration of this sequence in the soybean genome was confirmed by DNA deep sequencing. The locus where the integration occurred harbors the full RNA-1 sequence followed by the partial sequence of an endogenous mRNA and another sequence of RNA-1 as an inverted repeat and allowing the formation of a hairpin structure. This region recombined into a retrotransposon located inside an exon of a soybean gene. The nucleotide similarity of the integrated sequence compared to other Cucumber mosaic virus sequences indicates that the integration event occurred recently. We described a rare event of non-retroviral RNA virus integration in soybean that leads to the production of a double-stranded RNA in a similar fashion to virus resistance RNAi plants.
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01-internacional
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MEDLINE
Assunto principal:
Vírus de Plantas
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Glycine max
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Integração Viral
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RNA de Plantas
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Genoma de Planta
Idioma:
En
Revista:
Plant Sci
Ano de publicação:
2016
Tipo de documento:
Article