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High level soluble expression and one-step purification of IBDV VP2 protein in Escherichia coli.
Jiang, Dawei; Liu, Yunchao; Wang, Aiping; Zhang, Gaiping; Yang, Guoyu; Chen, Yumei; Ji, Pengchao; Liu, Chang; Song, Yapeng; Su, Yunfang; Wang, Guoqiang; Wang, Jucai; Zhao, Baolei; Deng, Ruiguang.
Afiliação
  • Jiang D; College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou, 450002, People's Republic of China.
  • Liu Y; Henan Provincial Key Laboratory of Animal Immunology, Henan Academy of Agricultural Sciences, Zhengzhou, 450002, People's Republic of China.
  • Wang A; Henan Provincial Key Laboratory of Animal Immunology, Henan Academy of Agricultural Sciences, Zhengzhou, 450002, People's Republic of China.
  • Zhang G; School of Life Sciences, Zhengzhou University, Zhengzhou, 450001, People's Republic of China.
  • Yang G; College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou, 450002, People's Republic of China. zhanggaiping2003@163.com.
  • Chen Y; Henan Provincial Key Laboratory of Animal Immunology, Henan Academy of Agricultural Sciences, Zhengzhou, 450002, People's Republic of China. zhanggaiping2003@163.com.
  • Ji P; College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou, 450002, People's Republic of China.
  • Liu C; Henan Provincial Key Laboratory of Animal Immunology, Henan Academy of Agricultural Sciences, Zhengzhou, 450002, People's Republic of China.
  • Song Y; School of Life Sciences, Zhengzhou University, Zhengzhou, 450001, People's Republic of China.
  • Su Y; Henan Provincial Key Laboratory of Animal Immunology, Henan Academy of Agricultural Sciences, Zhengzhou, 450002, People's Republic of China.
  • Wang G; Henan Provincial Key Laboratory of Animal Immunology, Henan Academy of Agricultural Sciences, Zhengzhou, 450002, People's Republic of China.
  • Wang J; College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou, 450002, People's Republic of China.
  • Zhao B; Henan Provincial Key Laboratory of Animal Immunology, Henan Academy of Agricultural Sciences, Zhengzhou, 450002, People's Republic of China.
  • Deng R; Henan Provincial Key Laboratory of Animal Immunology, Henan Academy of Agricultural Sciences, Zhengzhou, 450002, People's Republic of China.
Biotechnol Lett ; 38(6): 901-8, 2016 Jun.
Article em En | MEDLINE | ID: mdl-27003577
ABSTRACT

OBJECTIVES:

To improve the expression of soluble IBDV VP2 protein by using different tagged vectors in Escherichia coli.

RESULTS:

Fusion tags, Grifin, MBP, SUMO, thioredoxin, γ-crystallin, ArsC and PpiB, enhanced the expression and solubility of VP2 protein. The fusion proteins were purified by Ni-NTA chromatography, MBP-VP2 showed the highest purity about 90 %. After removing the MBP tag, VP2 self-assembled into virus-like particles, ~25 nm diam. Results from AGP suggested the recombinant IBDV VP2 protein identified by reference serum like IBDV.

CONCLUSION:

All the seven tags enhanced the expression and solubility of IBDV VP2 protein. The recombinant protein self-assembly into virus like particles and possess antigenicity as reference IBDV.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Proteínas Recombinantes de Fusão / Engenharia de Proteínas / Proteínas Estruturais Virais / Escherichia coli Limite: Animals Idioma: En Revista: Biotechnol Lett Ano de publicação: 2016 Tipo de documento: Article
Texto completo
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Proteínas Recombinantes de Fusão / Engenharia de Proteínas / Proteínas Estruturais Virais / Escherichia coli Limite: Animals Idioma: En Revista: Biotechnol Lett Ano de publicação: 2016 Tipo de documento: Article