Feasibility of blood flow measurement by externally controlled dye delivery.
Invest Ophthalmol Vis Sci
; 30(4): 660-7, 1989 Apr.
Article
em En
| MEDLINE
| ID: mdl-2703306
ABSTRACT
We are developing a new method of delivering substances locally and repeatedly in the retinal vasculature under external control. This delivery system is based on encapsulating the substance in heat-sensitive lipsomes, which are injected intravenously and lysed by a heat pulse delivered by a laser. The feasibility of using this system with dyes and creating a sharp dye front was tested in vitro and in vivo. The results indicate that the background fluorescence of intact liposomes is minimal but in contrast a dramatic increase in fluorescence is achieved where the dye is released. In vivo tests indicated that only the selected vascular branch fluoresced. Moreover, a sharp dye front could be obtained repeatedly and preserved over significant distances. The presence of a sharp dye front allowed measurements, in vitro, of blood velocity which correlated well (r = 0.985, P less than 0.001) with the average blood velocity values calculated from the known flow rate.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Vasos Retinianos
/
Corantes
Limite:
Animals
Idioma:
En
Revista:
Invest Ophthalmol Vis Sci
Ano de publicação:
1989
Tipo de documento:
Article