Differentiation and Characterization of Tr1 Cells.
Curr Protoc Immunol
; 113: 3.27.1-3.27.10, 2016 Apr 01.
Article
em En
| MEDLINE
| ID: mdl-27038462
ABSTRACT
Regulatory T cell-mediated suppression serves as a pivotal mechanism of negative regulation of immune-mediated inflammation. Type 1 regulatory T cells (Tr1 cells) are an important subset of CD4+ T cells that prevent excessive inflammatory responses and maintain immune tolerance. The anti-inflammatory role of Tr1 cells is mediated in part by their production of interleukin 10 (IL-10), which dampens the function of both antigen-presenting cells and antigen-specific effector T cells. Additionally, Tr1 cells can kill effector and myeloid cells through the perforin-granzyme B pathway. Adoptive transfer of in vitro differentiated Tr1 cells can be used to suppress autoimmune tissue inflammation in vivo. This unit describes the in vitro stimulation of naïve murine CD4+ T cells using IL-27 to generate IL-10-producing Tr1 cells.
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Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Diferenciação Celular
/
Subpopulações de Linfócitos T
/
Linfócitos T Reguladores
Limite:
Animals
Idioma:
En
Revista:
Curr Protoc Immunol
Ano de publicação:
2016
Tipo de documento:
Article