Development, validation and quantitative assessment of an enzymatic assay suitable for small molecule screening and profiling: A case-study.

Sancenon, Vicente; Goh, Wei Hau; Sundaram, Aishwarya; Er, Kai Shih; Johal, Nidhi; Mukhina, Svetlana; Carr, Grant; Dhakshinamoorthy, Saravanakumar

Sancenon, Vicente; Goh, Wei Hau; Sundaram, Aishwarya; Er, Kai Shih; Johal, Nidhi; Mukhina, Svetlana; Carr, Grant; Dhakshinamoorthy, Saravanakumar.

Afiliação

Sancenon V; Albany Molecular Research Singapore Research Centre, Pte Ltd, The Galen #05-01, 61 Science Park Road, Singapore 117525, Singapore.
Goh WH; Albany Molecular Research Singapore Research Centre, Pte Ltd, The Galen #05-01, 61 Science Park Road, Singapore 117525, Singapore.
Sundaram A; Albany Molecular Research Singapore Research Centre, Pte Ltd, The Galen #05-01, 61 Science Park Road, Singapore 117525, Singapore.
Er KS; Albany Molecular Research Singapore Research Centre, Pte Ltd, The Galen #05-01, 61 Science Park Road, Singapore 117525, Singapore.
Johal N; Albany Molecular Research Singapore Research Centre, Pte Ltd, The Galen #05-01, 61 Science Park Road, Singapore 117525, Singapore.
Mukhina S; Albany Molecular Research Singapore Research Centre, Pte Ltd, The Galen #05-01, 61 Science Park Road, Singapore 117525, Singapore.
Carr G; Albany Molecular Research Singapore Research Centre, Pte Ltd, The Galen #05-01, 61 Science Park Road, Singapore 117525, Singapore.
Dhakshinamoorthy S; Albany Molecular Research Singapore Research Centre, Pte Ltd, The Galen #05-01, 61 Science Park Road, Singapore 117525, Singapore.

Biomol Detect Quantif ; 4: 1-9, 2015 Jun.

Article em En | MEDLINE | ID: mdl-27077032

Palavras-chave

AP, alkaline phosphatase; Assay development; CV, coefficient of variation; DEA, diethanolamine; DiFMU, 6,8-difluoro-4-methylumbelliferone; DiFMUP, 6,8-difluoro-4-methylumbelliferyl phosphate; Inhibitor; KM, Michaelis constant; Mechanism-of-action; Phosphatase; SD, standard deviation; Screening; Small molecule; Vmax, maximal reaction velocity; Z', Z prime; pNP, p-nitrophenol; pNPP, p-nitrophenol phosphate

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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Tipo de estudo: Diagnostic_studies / Screening_studies Idioma: En Revista: Biomol Detect Quantif Ano de publicação: 2015 Tipo de documento: Article País de afiliação: Singapura

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