Cigarette smoke but not electronic cigarette aerosol activates a stress response in human coronary artery endothelial cells in culture.
Drug Alcohol Depend
; 163: 256-60, 2016 Jun 01.
Article
em En
| MEDLINE
| ID: mdl-27137404
ABSTRACT
BACKGROUND:
It is generally acknowledged that e-cigarettes are unlikely to be as harmful as conventional cigarettes, but there is little data that quantifies their relative harms. We investigated the biological response to e-cigarette aerosol exposure (versus conventional cigarette smoke exposure) at the cellular level, by exposing human coronary artery endothelial cells (HCAEC) to aqueous filtered extracts of e-cigarette aerosol or cigarette smoke and looking at gene expression changes consistent with a stress response. This included genes controlled by the oxidant-stress sensing transcription factor NFR2 (NFE2L2), and cytochrome P450 family members.METHODS:
Cigarette smoke extract (CSE) was created using mainstream smoke from a single cigarette drawn through 10ml of endothelial cell growth media MV2. Electronic cigarette aerosol extract (eCAE) was created using the same apparatus, using a constant power output of 10.8w (4.2V) and 18mg/ml nicotine solution. eCAE was generated using 5 cycles of 5s heat with at least 10s in between each puff to allow the coil to cool, air being drawn through the device at 70ml/minute.RESULTS:
HCAEC responded to the noxious components in CSE, resulting in activation of NRF2 and upregulation of cytochrome p450. However, eCAE did not induce NRF2 nuclear localisation, upregulation of NRF2-activated genes, or the upregulation of cytochrome p450.CONCLUSIONS:
The use of e-cigarettes as a substitute for conventional cigarettes is likely to reduce immediate tobacco-related harm, at least with respect to cardiovascular harms.Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Fumaça
/
Estresse Oxidativo
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Vasos Coronários
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Aerossóis
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Células Endoteliais
/
Sistemas Eletrônicos de Liberação de Nicotina
Limite:
Humans
Idioma:
En
Revista:
Drug Alcohol Depend
Ano de publicação:
2016
Tipo de documento:
Article
País de afiliação:
Reino Unido