Prokaryotic Soluble Overexpression and Purification of Human VEGF165 by Fusion to a Maltose Binding Protein Tag.

Nguyen, Minh Tan; Krupa, Martin; Koo, Bon-Kyung; Song, Jung-A; Vu, Thu Trang Thi; Do, Bich Hang; Nguyen, Anh Ngoc; Seo, Taewook; Yoo, Jiwon; Jeong, Boram; Jin, Jonghwa; Lee, Kyung Jin; Oh, Heung-Bum; Choe, Han

Nguyen, Minh Tan; Krupa, Martin; Koo, Bon-Kyung; Song, Jung-A; Vu, Thu Trang Thi; Do, Bich Hang; Nguyen, Anh Ngoc; Seo, Taewook; Yoo, Jiwon; Jeong, Boram; Jin, Jonghwa; Lee, Kyung Jin; Oh, Heung-Bum; Choe, Han.

Afiliação

Nguyen MT; Department of Physiology and Bio-Medical Institute of Technology, University of Ulsan College of Medicine, Asan Medical Center, Seoul 05505, Korea.
Krupa M; Department of Physiology and Bio-Medical Institute of Technology, University of Ulsan College of Medicine, Asan Medical Center, Seoul 05505, Korea.
Koo BK; Department of Physiology and Bio-Medical Institute of Technology, University of Ulsan College of Medicine, Asan Medical Center, Seoul 05505, Korea.
Song JA; Department of Physiology and Bio-Medical Institute of Technology, University of Ulsan College of Medicine, Asan Medical Center, Seoul 05505, Korea.
Vu TT; Department of Physiology and Bio-Medical Institute of Technology, University of Ulsan College of Medicine, Asan Medical Center, Seoul 05505, Korea.
Do BH; Department of Physiology and Bio-Medical Institute of Technology, University of Ulsan College of Medicine, Asan Medical Center, Seoul 05505, Korea.
Nguyen AN; Department of Physiology and Bio-Medical Institute of Technology, University of Ulsan College of Medicine, Asan Medical Center, Seoul 05505, Korea.
Seo T; Department of Physiology and Bio-Medical Institute of Technology, University of Ulsan College of Medicine, Asan Medical Center, Seoul 05505, Korea.
Yoo J; Department of Physiology and Bio-Medical Institute of Technology, University of Ulsan College of Medicine, Asan Medical Center, Seoul 05505, Korea.
Jeong B; Department of Physiology and Bio-Medical Institute of Technology, University of Ulsan College of Medicine, Asan Medical Center, Seoul 05505, Korea.
Jin J; Osong Medical Innovation Foundation, New Drug Development Center, Division of Drug Screening and Evaluation, Chungbuk, 363-951, Korea.
Lee KJ; Department of Convergence Medicine, Asan Institute for Life Sciences, University of Ulsan College of Medicine, Asan Medical Center, Seoul, 05505, Korea.
Oh HB; Department of Laboratory Medicine, University of Ulsan College of Medicine and Asan Medical Center, Seoul 05505, Korea.
Choe H; Department of Physiology and Bio-Medical Institute of Technology, University of Ulsan College of Medicine, Asan Medical Center, Seoul 05505, Korea.

PLoS One ; 11(5): e0156296, 2016.

Article em En | MEDLINE | ID: mdl-27231876

Assuntos

Escherichia coli/genética; Proteínas Ligantes de Maltose/genética; Proteínas Recombinantes de Fusão/genética; Proteínas Recombinantes de Fusão/isolamento & purificação; Fator A de Crescimento do Endotélio Vascular/genética; Fator A de Crescimento do Endotélio Vascular/isolamento & purificação; Animais; Células CHO; Cricetinae; Cricetulus; Expressão Gênica; Humanos; Plasmídeos/genética; Proteínas Recombinantes de Fusão/química; Solubilidade; Fator A de Crescimento do Endotélio Vascular/química

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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Proteínas Recombinantes de Fusão / Fator A de Crescimento do Endotélio Vascular / Escherichia coli / Proteínas Ligantes de Maltose Limite: Animals / Humans Idioma: En Revista: PLoS One Assunto da revista: CIENCIA / MEDICINA Ano de publicação: 2016 Tipo de documento: Article

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