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mRNA Electroporation of Dendritic Cells with WT1, Survivin, and TriMix (a Mixture of caTLR4, CD40L, and CD70).
Coosemans, An; Tuyaerts, Sandra; Morias, Kim; Corthals, Jurgen; Heirman, Carlo; Thielemans, Kris; Van Gool, Stefaan W; Vergote, Ignace; Amant, Frédéric.
Afiliação
  • Coosemans A; Laboratory of Gynecologic Oncology, Department of Oncology, KU Leuven, Leuven Cancer Institute, Leuven, Belgium. an.coosemans@gmail.com.
  • Tuyaerts S; Department of Gynecology and Obstetrics, UZ Leuven, Herestraat 49, 3000, Leuven, Belgium. an.coosemans@gmail.com.
  • Morias K; Laboratory of Gynecologic Oncology, Department of Oncology, KU Leuven, Leuven Cancer Institute, Leuven, Belgium.
  • Corthals J; Department of Human Genetics, KU Leuven, Leuven, Belgium.
  • Heirman C; Laboratory of Molecular and Cellular Therapy (LMCT), Vrije Universiteit Brussel, Brussels, Belgium.
  • Thielemans K; Laboratory of Molecular and Cellular Therapy (LMCT), Vrije Universiteit Brussel, Brussels, Belgium.
  • Van Gool SW; Laboratory of Molecular and Cellular Therapy (LMCT), Vrije Universiteit Brussel, Brussels, Belgium.
  • Vergote I; Laboratory of Pediatric Immunology, Department of Microbiology and Immunology, KU Leuven, Leuven, Belgium.
  • Amant F; Laboratory of Gynecologic Oncology, Department of Oncology, KU Leuven, Leuven Cancer Institute, Leuven, Belgium.
Methods Mol Biol ; 1428: 277-83, 2016.
Article em En | MEDLINE | ID: mdl-27236806
ABSTRACT
The immune system is a crucial player in the development of cancer. Once it is in imbalance and immunosuppressive mechanisms supporting tumor growth take over control, dendritic cell immunotherapy might offer a solution to restore the balance. There are several methods to manufacture dendritic cells but none of them has yet proven to be superior to others. In this chapter, we discuss the methodology using electroporation of mRNA encoding Wilms' tumor gene 1, survivin, and TriMix (mixture of caTLR4, CD40L, and CD70) to simultaneously load and mature dendritic cells.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Células Dendríticas / RNA Mensageiro / Eletroporação / Antígenos de Neoplasias Limite: Humans Idioma: En Revista: Methods Mol Biol Assunto da revista: BIOLOGIA MOLECULAR Ano de publicação: 2016 Tipo de documento: Article País de afiliação: Bélgica
Texto completo
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Células Dendríticas / RNA Mensageiro / Eletroporação / Antígenos de Neoplasias Limite: Humans Idioma: En Revista: Methods Mol Biol Assunto da revista: BIOLOGIA MOLECULAR Ano de publicação: 2016 Tipo de documento: Article País de afiliação: Bélgica