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Preparation and characterization of cationic and amphoteric mannans from Candida albicans.
Cízová, Alzbeta; Nescáková, Zuzana; Malovíková, Anna; Bystrický, Slavomír.
Afiliação
  • Cízová A; Institute of Chemistry, Slovak Academy of Sciences, Dúbravská cesta 9, 845 38 Bratislava, Slovak Republic. Electronic address: alzbeta.cizova@savba.sk.
  • Nescáková Z; Institute of Chemistry, Slovak Academy of Sciences, Dúbravská cesta 9, 845 38 Bratislava, Slovak Republic. Electronic address: zuzana.nescakova@gmail.com.
  • Malovíková A; Institute of Chemistry, Slovak Academy of Sciences, Dúbravská cesta 9, 845 38 Bratislava, Slovak Republic. Electronic address: anna.malovikova@savba.sk.
  • Bystrický S; Institute of Chemistry, Slovak Academy of Sciences, Dúbravská cesta 9, 845 38 Bratislava, Slovak Republic. Electronic address: slavomir.bystricky@savba.sk.
Carbohydr Polym ; 149: 1-7, 2016 09 20.
Article em En | MEDLINE | ID: mdl-27261724
Cationic and amphoteric mannans from Candida albicans were prepared by chemical modification with (3-chloro-2-hydroxypropyl)trimethylammonium chloride (CHPTAC) and sodium chloroacetate under aqueous alkaline conditions. The optimal reaction conditions for mannan cationization were found to be 6h, 60°C, and NaOH/CHPTAC ratio of 1.0. Adjusting the molar ratio of cationization agent to anhydromannose unit, cationic and amphoteric mannans with degree of substitution ranging from 0.07 to 0.57 were obtained. Their structure was confirmed by elemental analysis as well as FTIR and NMR spectroscopies. Moderate decrease of molecular weight of both cationic and amphoteric mannans was recorded by size exclusion chromatography. With increasing level of modification, reduction of the antibody-binding capacity was observed by enzyme-linked immunosorbent assay.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Candida albicans / Mananas Idioma: En Revista: Carbohydr Polym Ano de publicação: 2016 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Candida albicans / Mananas Idioma: En Revista: Carbohydr Polym Ano de publicação: 2016 Tipo de documento: Article