Severe muscle trauma triggers heightened and prolonged local musculoskeletal inflammation and impairs adjacent tibia fracture healing.
J Musculoskelet Neuronal Interact
; 16(2): 122-34, 2016 06 01.
Article
em En
| MEDLINE
| ID: mdl-27282456
ABSTRACT
OBJECTIVES:
Complicated fracture healing is often associated with the severity of surrounding muscle tissue trauma. Since inflammation is a primary determinant of musculoskeletal health and regeneration, it is plausible that delayed healing and non-unions are partly caused by compounding local inflammation in response to concomitant muscle trauma. METHODS ANDRESULTS:
To investigate this possibility, a Lewis rat open fracture model [tibia osteotomy with adjacent tibialis anterior (TA) muscle volumetric muscle loss (VML) injury] was interrogated. We observed that VML injury impaired tibia healing, as indicated by diminished mechanical strength and decreased mineralized bone within the fracture callus, as well as continued presence of cartilage instead of woven bone 28 days post-injury. The VML injured muscle presented innate and adaptive immune responses that were atypical of canonical muscle injury healing. Additionally, the VML injury resulted in a perturbation of the inflammatory phase of fracture healing, as indicated by elevations of CD3(+) lymphocytes and CD68+ macrophages in the fracture callus at 3 and 14d post-injury, respectively.CONCLUSIONS:
These data indicate that heightened and sustained innate and adaptive immune responses to traumatized muscle are associated with impaired fracture healing and may be targeted for the prevention of delayed and non-union following musculoskeletal trauma.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Fraturas da Tíbia
/
Consolidação da Fratura
/
Músculo Esquelético
/
Fraturas Expostas
/
Inflamação
Limite:
Animals
Idioma:
En
Revista:
J Musculoskelet Neuronal Interact
Assunto da revista:
FISIOLOGIA
/
NEUROLOGIA
/
ORTOPEDIA
Ano de publicação:
2016
Tipo de documento:
Article