Brain region-specific effects of cGMP-dependent kinase II knockout on AMPA receptor trafficking and animal behavior.
Learn Mem
; 23(8): 435-41, 2016 08.
Article
em En
| MEDLINE
| ID: mdl-27421896
ABSTRACT
Phosphorylation of GluA1, a subunit of AMPA receptors (AMPARs), is critical for AMPAR synaptic trafficking and control of synaptic transmission. cGMP-dependent protein kinase II (cGKII) mediates this phosphorylation, and cGKII knockout (KO) affects GluA1 phosphorylation and alters animal behavior. Notably, GluA1 phosphorylation in the KO hippocampus is increased as a functional compensation for gene deletion, while such compensation is absent in the prefrontal cortex. Thus, there are brain region-specific effects of cGKII KO on AMPAR trafficking, which could affect animal behavior. Here, we show that GluA1 phosphorylation levels differ in various brain regions, and specific behaviors are altered according to region-specific changes in GluA1 phosphorylation. Moreover, we identified distinct regulations of phosphatases in different brain regions, leading to regional heterogeneity of GluA1 phosphorylation in the KO brain. Our work demonstrates region-specific changes in GluA1 phosphorylation in cGKII KO mice and corresponding effects on cognitive performance. We also reveal distinct regulation of phosphatases in different brain region in which region-specific effects of kinase gene KO arise and can selectively alter animal behavior.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Comportamento Animal
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Encéfalo
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Receptores de AMPA
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Transporte Proteico
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Proteína Quinase Dependente de GMP Cíclico Tipo II
Limite:
Animals
Idioma:
En
Revista:
Learn Mem
Assunto da revista:
NEUROLOGIA
Ano de publicação:
2016
Tipo de documento:
Article