Id3 Orchestrates Germinal Center B Cell Development.
Mol Cell Biol
; 36(20): 2543-52, 2016 10 15.
Article
em En
| MEDLINE
| ID: mdl-27457619
ABSTRACT
Previous studies have demonstrated that E proteins induce activation-induced deaminase (AID) expression in activated B cells. Here, we examined the role of Id3 in germinal center (GC) cells. We found that Id3 expression is high in follicular B lineage cells but declines in GC cells. Immunized mice with Id3 expression depleted displayed a block in germinal center B cell maturation, showed reduced numbers of marginal zone B cells and class-switched cells, and were associated with decreased antibody titers and lower numbers of plasma cells. In vitro, Id3-depleted B cells displayed a defect in class switch recombination. Whereas AID levels were not altered in Id3-depleted activated B cells, the expression of a subset of genes encoding signaling components of antigen receptor-, cytokine receptor-, and chemokine receptor-mediated signaling was significantly impaired. We propose that during the GC reaction, Id3 levels decline to activate the expression of genes encoding signaling components that mediate B cell receptor- and or cytokine receptor-mediated signaling to promote the differentiation of GC B cells.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Linfócitos B
/
Imunização
/
Centro Germinativo
/
Citidina Desaminase
/
Proteínas Inibidoras de Diferenciação
Limite:
Animals
Idioma:
En
Revista:
Mol Cell Biol
Ano de publicação:
2016
Tipo de documento:
Article
País de afiliação:
Estados Unidos