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Analgesic and anti-inflammatory actions on bradykinin route of a polysulfated fraction from alga Ulva lactuca.
de Araújo, Ianna Wivianne Fernandes; Rodrigues, José Ariévilo Gurgel; Quinderé, Ana Luíza Gomes; Silva, Jane de Fátima Teixeira; Maciel, Gabrielle de Freitas; Ribeiro, Natássia Albuquerque; de Sousa Oliveira Vanderlei, Edfranck; Ribeiro, Kátia Alves; Chaves, Hellíada Vasconcelos; Pereira, Karuza Maria Alves; Bezerra, Mirna Marques; Benevides, Norma Maria Barros.
Afiliação
  • de Araújo IW; Departamento de Engenharia de Pesca, Universidade Federal do Ceará, Avenida Humberto Monte, s/n, Campus do Pici, CEP: 60455-760, Fortaleza, Brazil. Electronic address: iwfaraujo@gmail.com.
  • Rodrigues JA; Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Ceará, Avenida Humberto Monte, s/n, Campus do Pici, CEP: 60455-760, Fortaleza, Brazil.
  • Quinderé AL; Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Ceará, Avenida Humberto Monte, s/n, Campus do Pici, CEP: 60455-760, Fortaleza, Brazil.
  • Silva JF; Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Ceará, Avenida Humberto Monte, s/n, Campus do Pici, CEP: 60455-760, Fortaleza, Brazil.
  • Maciel GF; Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Ceará, Avenida Humberto Monte, s/n, Campus do Pici, CEP: 60455-760, Fortaleza, Brazil.
  • Ribeiro NA; Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Ceará, Avenida Humberto Monte, s/n, Campus do Pici, CEP: 60455-760, Fortaleza, Brazil.
  • de Sousa Oliveira Vanderlei E; Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Ceará, Avenida Humberto Monte, s/n, Campus do Pici, CEP: 60455-760, Fortaleza, Brazil.
  • Ribeiro KA; Faculdade de Medicina, Universidade Federal do Ceará, Rua Coronel Estanislau Frota, s/n-Centro, Campus Sobral, CEP: 62010-560, Sobral, Brazil.
  • Chaves HV; Faculdade de Odontologia, Universidade Federal do Ceará, Rua Coronel Estanislau Frota, s/n-Centro, Campus Sobral, CEP: 62010-560, Sobral, Brazil.
  • Pereira KM; Faculdade de Odontologia, Universidade Federal do Ceará, Rua Coronel Estanislau Frota, s/n-Centro, Campus Sobral, CEP: 62010-560, Sobral, Brazil.
  • Bezerra MM; Faculdade de Medicina, Universidade Federal do Ceará, Rua Coronel Estanislau Frota, s/n-Centro, Campus Sobral, CEP: 62010-560, Sobral, Brazil.
  • Benevides NM; Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Ceará, Avenida Humberto Monte, s/n, Campus do Pici, CEP: 60455-760, Fortaleza, Brazil. Electronic address: nmbb@ufc.br.
Int J Biol Macromol ; 92: 820-830, 2016 Nov.
Article em En | MEDLINE | ID: mdl-27481342
ABSTRACT
We investigated structural features of polysaccharides from Ulva lactuca and their effects on the classical models of nociception and inflammation. Crude extract was obtained by enzymatic digestion and isolated by ion exchange chromatography on DEAE-cellulose. The fraction with higher yield was used in the tests (SP-Ul). Swiss mice received SP-Ul (1, 3 or 9mg/kg; i.v.), 30min prior to injection of 0.8%-acetic acid or 1%-formalin or prior to a thermal stimulus. At same doses, SP-Ul was tested on Wistar rats on paw edema elicited by different irritants (carrageenan, dextran, bradykinin, histamine or serotonin). The results of infrared characterization indicated the presence of hydroxyl groups, sulfate, uronic acid and glycosidic linkages in all SP fractions spectrums. SP-Ul decreased significantly the antinociception in response to acetic acid or formalin (second phase), but not in the hot-plate test, suggesting that its analgesia occurs through a peripheral mechanism. SP-Ul did not reduce carrageenan-induced paw edema as supported by both histological and myeloperoxidase activity assessments. However, SP-Ul (1mg/kg; s.c.) reduced dextran-elicited edema, showing vascular anti-inflammatory effect, with bradykinin as major target because it did not reduce histamine- and serotonin-induced paw edemas. Therefore, SP-Ul acts on bradykinin pathway in its antinociceptive and anti-inflammatory responses.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Dor / Polissacarídeos / Bradicinina / Ulva / Edema / Analgésicos / Anti-Inflamatórios Limite: Animals Idioma: En Revista: Int J Biol Macromol Ano de publicação: 2016 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Dor / Polissacarídeos / Bradicinina / Ulva / Edema / Analgésicos / Anti-Inflamatórios Limite: Animals Idioma: En Revista: Int J Biol Macromol Ano de publicação: 2016 Tipo de documento: Article