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Polymer microfiber meshes facilitate cardiac differentiation of c-kit(+) human cardiac stem cells.
Kan, Lijuan; Thayer, Patrick; Fan, Huimin; Ledford, Benjamin; Chen, Miao; Goldstein, Aaron; Cao, Guohua; He, Jia-Qiang.
Afiliação
  • Kan L; Department of Biomedical Sciences and Pathobiology, Virginia-Maryland Regional College of Veterinary Medicine, Virginia Tech, Blacksburg, VA, United States.
  • Thayer P; Department of Chemical Engineering, School of Biomedical Engineering and Sciences, Virginia Tech, Blacksburg, VA, United States.
  • Fan H; Research Institute of Heart Failure, Shanghai East Hospital of Tongji University, Shanghai, PR China.
  • Ledford B; Department of Biomedical Sciences and Pathobiology, Virginia-Maryland Regional College of Veterinary Medicine, Virginia Tech, Blacksburg, VA, United States.
  • Chen M; Department of Biomedical Sciences and Pathobiology, Virginia-Maryland Regional College of Veterinary Medicine, Virginia Tech, Blacksburg, VA, United States.
  • Goldstein A; Department of Chemical Engineering, School of Biomedical Engineering and Sciences, Virginia Tech, Blacksburg, VA, United States.
  • Cao G; School of Biomedical Engineering and Sciences, Virginia Tech, Blacksburg, VA, United States.
  • He JQ; Department of Biomedical Sciences and Pathobiology, Virginia-Maryland Regional College of Veterinary Medicine, Virginia Tech, Blacksburg, VA, United States. Electronic address: jiahe@vt.edu.
Exp Cell Res ; 347(1): 143-152, 2016 09 10.
Article em En | MEDLINE | ID: mdl-27481582
Electrospun microfiber meshes have been shown to support the proliferation and differentiation of many types of stem cells, but the phenotypic fate of c-kit(+) human cardiac stem cells (hCSCs) have not been explored. To this end, we utilized thin (~5µm) elastomeric meshes consisting of aligned 1.7µm diameter poly (ester-urethane urea) microfibers as substrates to examine their effect on hCSC viability, morphology, proliferation, and differentiation relative to cells cultured on tissue culture polystyrene (TCPS). The results showed that cells on microfiber meshes displayed an elongated morphology aligned in the direction of fiber orientation, lower proliferation rates, but increased expressions of genes and proteins majorly associated with cardiomyocyte phenotype. The early (NK2 homeobox 5, Nkx2.5) and late (cardiac troponin I, cTnI) cardiomyocyte genes were significantly increased on meshes (Nkx=2.5 56.2±13.0, cTnl=2.9±0.56,) over TCPS (Nkx2.5=4.2±0.9, cTnl=1.6±0.5, n=9, p<0.05 for both groups) after differentiation. In contrast, expressions of smooth muscle markers, Gata6 and myosin heavy chain (SM-MHC), were decreased on meshes. Immunocytochemical analysis with cardiac antibody exhibited the similar pattern of above cardiac differentiation. We conclude that aligned microfiber meshes are suitable for guiding cardiac differentiation of hCSCs and may facilitate stem cell-based therapies for treatment of cardiac diseases.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Poliésteres / Células-Tronco / Diferenciação Celular / Proteínas Proto-Oncogênicas c-kit / Alicerces Teciduais / Miocárdio Limite: Humans Idioma: En Revista: Exp Cell Res Ano de publicação: 2016 Tipo de documento: Article País de afiliação: Estados Unidos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Poliésteres / Células-Tronco / Diferenciação Celular / Proteínas Proto-Oncogênicas c-kit / Alicerces Teciduais / Miocárdio Limite: Humans Idioma: En Revista: Exp Cell Res Ano de publicação: 2016 Tipo de documento: Article País de afiliação: Estados Unidos