Scoping the effectiveness and evolutionary obstacles in using plasmid-dependent phages to fight antibiotic resistance.
Future Microbiol
; 11: 999-1009, 2016 08.
Article
em En
| MEDLINE
| ID: mdl-27503765
ABSTRACT
AIM:
To investigate the potential evolutionary obstacles in the sustainable therapeutic use of plasmid-dependent phages to control the clinically important conjugative plasmid-mediated dissemination of antibiotic resistance genes to pathogenic bacteria. MATERIALS &METHODS:
The lytic plasmid-dependent phage PRD1 and the multiresistance conferring plasmid RP4 in an Escherichia coli host were utilized to assess the genetic and phenotypic changes induced by combined phage and antibiotic selection. RESULTS &CONCLUSIONS:
Resistance to PRD1 was always coupled with either completely lost or greatly reduced conjugation ability. Reversion to full conjugation efficiency was found to be rare, and it also restored the susceptibility to plasmid-dependent phages. Consequently, plasmid-dependent phages constitute an interesting candidate for development of sustainable anticonjugation/antiresistance therapeutic applications.Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Plasmídeos
/
Bacteriófagos
/
Farmacorresistência Bacteriana
/
Escherichia coli
/
Infecções por Escherichia coli
/
Antibacterianos
Limite:
Humans
Idioma:
En
Revista:
Future Microbiol
Assunto da revista:
MICROBIOLOGIA
Ano de publicação:
2016
Tipo de documento:
Article
País de afiliação:
Finlândia