Similar GABAA receptor subunit composition in somatic and axon initial segment synapses of hippocampal pyramidal cells.
Elife
; 52016 08 18.
Article
em En
| MEDLINE
| ID: mdl-27537197
ABSTRACT
Hippocampal pyramidal cells (PCs) express many GABAAR subunit types and receive GABAergic inputs from distinct interneurons. Previous experiments revealed input-specific differences in α1 and α2 subunit densities in perisomatic synapses, suggesting distinct IPSC decay kinetics. However, IPSC decays evoked by axo-axonic, parvalbumin- or cholecystokinin-expressing basket cells were found to be similar. Using replica immunogold labeling, here we show that all CA1 PC somatic and AIS synapses contain the α1, α2, ß1, ß2, ß3 and γ2 subunits. In CA3 PCs, 90% of the perisomatic synapses are immunopositive for the α1 subunit and all synapses are positive for the remaining five subunits. Somatic synapses form unimodal distributions based on their immunoreactivity for these subunits. The α2 subunit densities in somatic synapses facing Cav2.1 (i.e. parvalbumin) or Cav2.2 (cholecystokinin) positive presynaptic active zones are comparable. We conclude that perisomatic synapses made by three distinct interneuron types have similar GABAA receptor subunit content.
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Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Sinapses
/
Receptores de GABA
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Células Piramidais
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Subunidades Proteicas
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Segmento Inicial do Axônio
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Hipocampo
/
Interneurônios
Limite:
Animals
Idioma:
En
Revista:
Elife
Ano de publicação:
2016
Tipo de documento:
Article
País de afiliação:
Hungria