Control of early HIV-1 infection associates with plasmacytoid dendritic cell-reactive opsonophagocytic IgG antibodies to HIV-1 p24.
AIDS
; 30(18): 2757-2765, 2016 11 28.
Article
em En
| MEDLINE
| ID: mdl-27603291
ABSTRACT
OBJECTIVES:
We have previously demonstrated that HIV-1 p24-specific plasmacytoid dendritic cell-reactive opsonophagocytic antibody (PROAb) responses associate with control of chronic HIV infection. Here, we examined whether HIV-1 p24-specific PROAbs associate with control of early HIV infection and their relationship with HIV-1 p24-specific IgG subclasses.METHODS:
Plasma collected at 8 and 52 weeks following primary HIV-1 infection was obtained from antiretroviral therapy-naïve patients who were classified as 'good' (plasma HIV-1 RNAâ<â5000 copies/ml; nâ=â17) or 'poor' (HIV-1 RNAâ>â50â000 copies/ml; nâ=â15) controllers at week 52. HIV-1 p24-specific PROAb responses were assayed using a plasmacytoid dendritic cell line (Gen2.2), and HIV-1 p24-specific IgG3, IgG1 and IgG2 levels were assayed by ELISA.RESULTS:
HIV-1 p24-specific PROAb responses increased in 'good controllers' (Pâ=â0.01) but remained unchanged in 'poor controllers' between weeks 8 and 52. Of the HIV-1 p24-specific IgG subclasses measured, only IgG1 increased over this time period in 'good controllers' alone (Pâ=â0.003), which correlated with the increase in HIV-1 p24-specific PROAb responses (râ=â0.83, Pâ<â0.0001). Depletion of IgG1 from IgG preparations of 'good controllers' resulted in the inhibition of HIV-1 p24-specific PROAb responses. In the total patient cohort, plasma HIV-1 RNA levels at week 52 correlated inversely with changes in HIV-1 p24-specific PROAb responses (râ=â-0.37, Pâ=â0.04) and IgG1 (râ=â-0.51, Pâ=â0.003) levels between weeks 8 and 52.CONCLUSION:
Control of early HIV-1 infection was associated with an increase in HIV-1 p24-specific PROAb responses, which was mediated by HIV-1 p24-specific IgG1 antibodies. These findings provide further evidence that antibodies to HIV core proteins may contribute to control of HIV-1 infection.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Fagocitose
/
Células Dendríticas
/
Imunoglobulina G
/
Proteínas Opsonizantes
/
Anticorpos Anti-HIV
/
Infecções por HIV
/
Proteína do Núcleo p24 do HIV
Tipo de estudo:
Risk_factors_studies
Limite:
Adult
/
Humans
Idioma:
En
Revista:
AIDS
Assunto da revista:
SINDROME DA IMUNODEFICIENCIA ADQUIRIDA (AIDS)
Ano de publicação:
2016
Tipo de documento:
Article
País de afiliação:
Austrália