Probing the metabolic heterogeneity of live Euglena gracilis with stimulated Raman scattering microscopy.
Nat Microbiol
; 1(10): 16124, 2016 08 01.
Article
em En
| MEDLINE
| ID: mdl-27670110
ABSTRACT
Understanding metabolism in live microalgae is crucial for efficient biomaterial engineering, but conventional methods fail to evaluate heterogeneous populations of motile microalgae due to the labelling requirements and limited imaging speeds. Here, we demonstrate label-free video-rate metabolite imaging of live Euglena gracilis and statistical analysis of intracellular metabolite distributions under different culture conditions. Our approach provides further insights into understanding microalgal heterogeneity, optimizing culture methods and screening mutant microalgae.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Análise Espectral Raman
/
Água
/
Euglena gracilis
Limite:
Animals
Idioma:
En
Revista:
Nat Microbiol
Ano de publicação:
2016
Tipo de documento:
Article
País de afiliação:
Japão