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Fluorescent indicators for simultaneous reporting of all four cell cycle phases.
Bajar, Bryce T; Lam, Amy J; Badiee, Ryan K; Oh, Young-Hee; Chu, Jun; Zhou, Xin X; Kim, Namdoo; Kim, Benjamin B; Chung, Mingyu; Yablonovitch, Arielle L; Cruz, Barney F; Kulalert, Kanokwan; Tao, Jacqueline J; Meyer, Tobias; Su, Xiao-Dong; Lin, Michael Z.
Afiliação
  • Bajar BT; Department of Bioengineering, Stanford University, Stanford, California, USA.
  • Lam AJ; Department of Pediatrics, Stanford University, Stanford, California, USA.
  • Badiee RK; School of Life Sciences, Peking University, Beijing, China.
  • Oh YH; Department of Bioengineering, Stanford University, Stanford, California, USA.
  • Chu J; Department of Pediatrics, Stanford University, Stanford, California, USA.
  • Zhou XX; Department of Biological Sciences, Stanford University, Stanford, California, USA.
  • Kim N; Department of Bioengineering, Stanford University, Stanford, California, USA.
  • Kim BB; Department of Pediatrics, Stanford University, Stanford, California, USA.
  • Chung M; Department of Neurobiology, Stanford University, Stanford, California, USA.
  • Yablonovitch AL; Department of Bioengineering, Stanford University, Stanford, California, USA.
  • Cruz BF; Department of Pediatrics, Stanford University, Stanford, California, USA.
  • Kulalert K; Department of Bioengineering, Stanford University, Stanford, California, USA.
  • Tao JJ; Department of Pediatrics, Stanford University, Stanford, California, USA.
  • Meyer T; Department of Neurobiology, Stanford University, Stanford, California, USA.
  • Su XD; Department of Bioengineering, Stanford University, Stanford, California, USA.
  • Lin MZ; Department of Pediatrics, Stanford University, Stanford, California, USA.
Nat Methods ; 13(12): 993-996, 2016 Dec.
Article em En | MEDLINE | ID: mdl-27798610
A robust method for simultaneous visualization of all four cell cycle phases in living cells is highly desirable. We developed an intensiometric reporter of the transition from S to G2 phase and engineered a far-red fluorescent protein, mMaroon1, to visualize chromatin condensation in mitosis. We combined these new reporters with the previously described Fucci system to create Fucci4, a set of four orthogonal fluorescent indicators that together resolve all cell cycle phases.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Proteínas Recombinantes de Fusão / Ciclo Celular / Imagem Molecular / Imagem com Lapso de Tempo / Proteínas Luminescentes Limite: Animals / Humans Idioma: En Revista: Nat Methods Assunto da revista: TECNICAS E PROCEDIMENTOS DE LABORATORIO Ano de publicação: 2016 Tipo de documento: Article País de afiliação: Estados Unidos
Texto completo
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Proteínas Recombinantes de Fusão / Ciclo Celular / Imagem Molecular / Imagem com Lapso de Tempo / Proteínas Luminescentes Limite: Animals / Humans Idioma: En Revista: Nat Methods Assunto da revista: TECNICAS E PROCEDIMENTOS DE LABORATORIO Ano de publicação: 2016 Tipo de documento: Article País de afiliação: Estados Unidos