Blue Native Protein Electrophoresis to Study the T3S System Using Yersinia pestis as a Model.
Methods Mol Biol
; 1531: 33-46, 2017.
Article
em En
| MEDLINE
| ID: mdl-27837479
ABSTRACT
Since the introduction of blue native, clear native, and high-resolution clear native electrophoresis to study protein complexes of eukaryotic, bacterial, and archaeal cells, the technique has been used primarily to study physiological systems that are found in abundance within the cell. Systems involved in oxidative phosphorylation, electron transport, membrane transporters, and secretion systems have been studied using these techniques. These microscale techniques are ideal due to the minimal perturbations caused to these protein complexes. The utility of the blue native electrophoresis method was determined in a study described here of protein complexes identified in the plague causing bacteria, Yersinia pestis. In addition, the technique was used to observe how LcrG, a negative regulator of the pathogenic Type III secretion system (T3SS), interacts with the T3SS and other protein complexes.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Yersinia pestis
/
Eletroforese
/
Sistemas de Secreção Tipo III
Idioma:
En
Revista:
Methods Mol Biol
Assunto da revista:
BIOLOGIA MOLECULAR
Ano de publicação:
2017
Tipo de documento:
Article
País de afiliação:
Estados Unidos