Role of perivascular adipose tissue in nicotineinduced endothelial cell inflammatory responses.
Mol Med Rep
; 14(6): 5713-5718, 2016 Dec.
Article
em En
| MEDLINE
| ID: mdl-27840948
ABSTRACT
Smoking is considered to be one of the primary causes of atherosclerosis and vascular injury. Previous studies have shown that nicotine in tobacco can lead to vascular inflammation and endothelial dysfunction. Perivascular adipose tissue (PVAT) is known to secrete various types of adipokines to maintain vascular homeostasis. The present study investigated whether nicotineinduced PVAT malfunction can accelerate endothelial inflammation and eventually lead to endothelial dysfunction. The levels of inflammatory adipokines, including nuclear factor (NF)κB, interleukin (IL)1ß, IL6 and tumor necrosis factor (TNF)α, the ICAM1 and VCAM1 adhesion molecules and secretion of adiponectin were assessed in mature adipocytes and endothelial cells cultured alone or in coculture under nicotine stimulation. It was found that nicotine reduced the secretion of adiponectin and stimulated secretion of the NFκB, IL1ß, IL6 and TNFα inflammatory adipokines in mature adipocytes. Although nicotine stimulated endothelial cells to secrete IL1ß and IL6, no significant increase in the secretion of TNFα was observed. The coculture of mature adipocytes with endothelial cells markedly augmented the expression of the NFκB, IL1ß, IL6 and TNFα inflammatory adipokines and the ICAM1 and VCAM1 adhesion molecules, and significantly lowered the levels of adiponectin. These findings suggested that nicotine induced mature adipocyte dysfunction, which caused the abnormal secretion of adiponectin and inflammatory adipokines, and exacerbated endothelial inflammation. These findings also suggested a mechanism whereby nicotine induced the secretion of adiponectin and inflammatory cytokines by adipocytes. The results of the present study elucidated a novel pathway induced by cigarette smoke, which contributed to atherosclerosis and vascular injury.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Endotélio Vascular
/
Tecido Adiposo
/
Células Endoteliais
/
Nicotina
Tipo de estudo:
Etiology_studies
Limite:
Animals
/
Humans
Idioma:
En
Revista:
Mol Med Rep
Ano de publicação:
2016
Tipo de documento:
Article