Exploring the Role of PGC-1α in Defining Nuclear Organisation in Skeletal Muscle Fibres.
J Cell Physiol
; 232(6): 1270-1274, 2017 Jun.
Article
em En
| MEDLINE
| ID: mdl-27861863
ABSTRACT
Muscle fibres are multinucleated cells, with each nucleus controlling the protein synthesis in a finite volume of cytoplasm termed the myonuclear domain (MND). What determines MND size remains unclear. In the present study, we aimed to test the hypothesis that the level of expression of the transcriptional coactivator PGC-1α and subsequent activation of the mitochondrial biogenesis are major contributors. Hence, we used two transgenic mouse models with varying expression of PGC-1α in skeletal muscles. We isolated myofibres from the fast twitch extensor digitorum longus (EDL) and slow twitch diaphragm muscles. We then membrane-permeabilised them and analysed the 3D spatial arrangements of myonuclei. In EDL muscles, when PGC-1α is over-expressed, MND volume decreases; whereas, when PGC-1α is lacking, no change occurs. In the diaphragm, no clear difference was noted. This indicates that PGC-1α and the related mitochondrial biogenesis programme are determinants of MND size. PGC-1α may facilitate the addition of new myonuclei in order to reach MND volumes that can support an increased mitochondrial density. J. Cell. Physiol. 232 1270-1274, 2017. © 2016 Wiley Periodicals, Inc.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Núcleo Celular
/
Fibras Musculares Esqueléticas
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Coativador 1-alfa do Receptor gama Ativado por Proliferador de Peroxissomo
Limite:
Animals
Idioma:
En
Revista:
J Cell Physiol
Ano de publicação:
2017
Tipo de documento:
Article
País de afiliação:
Reino Unido