Influence of antibiotics on motility and adherence of human neutrophils studied in vitro.

ABSTRACT

The influence of 14 antibiotics on chemotaxis, motility and adherence of human neutrophils has been studied. Neutrophils were preincubated for 30 min at 37 degrees C with the antibiotics. Chemotaxis was studied in antibiotic-free and antibiotic-containing agar using zymosan-activated serum and fMLP as stimuli. Adherence was studied in plastic microtitre plates. Most of the antibiotics studied did not affect the three functions studied. Daptomycin occasionally decreased random migration without affecting chemotaxis and adherence. Chemotaxis was occasionally modified by some of the quinolones, although this depended on the volunteer tested. The level of inhibition could be significant in diseased patients.