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Molecular profiling of single circulating tumor cells from lung cancer patients.
Park, Seung-Min; Wong, Dawson J; Ooi, Chin Chun; Kurtz, David M; Vermesh, Ophir; Aalipour, Amin; Suh, Susie; Pian, Kelsey L; Chabon, Jacob J; Lee, Sang Hun; Jamali, Mehran; Say, Carmen; Carter, Justin N; Lee, Luke P; Kuschner, Ware G; Schwartz, Erich J; Shrager, Joseph B; Neal, Joel W; Wakelee, Heather A; Diehn, Maximilian; Nair, Viswam S; Wang, Shan X; Gambhir, Sanjiv S.
Afiliação
  • Park SM; Department of Radiology, Stanford University School of Medicine, Stanford, CA 94305.
  • Wong DJ; Molecular Imaging Program at Stanford, Stanford University School of Medicine, Stanford, CA 94305.
  • Ooi CC; Department of Electrical Engineering, Stanford University, Stanford, CA 94305.
  • Kurtz DM; Department of Chemical Engineering, Stanford University, Stanford, CA 94305.
  • Vermesh O; Molecular Imaging Program at Stanford, Stanford University School of Medicine, Stanford, CA 94305.
  • Aalipour A; Department of Bioengineering, Stanford University, Stanford, CA 94305.
  • Suh S; Division of Oncology, Department of Medicine, Stanford University School of Medicine, Stanford, CA 94305.
  • Pian KL; Department of Radiology, Stanford University School of Medicine, Stanford, CA 94305.
  • Chabon JJ; Molecular Imaging Program at Stanford, Stanford University School of Medicine, Stanford, CA 94305.
  • Lee SH; Department of Radiology, Stanford University School of Medicine, Stanford, CA 94305.
  • Jamali M; Molecular Imaging Program at Stanford, Stanford University School of Medicine, Stanford, CA 94305.
  • Say C; Department of Pharmacology, Case Western Reserve University School of Medicine, Cleveland, OH 44106.
  • Carter JN; Department of Materials Science and Engineering, Stanford University, Stanford, CA 94305.
  • Lee LP; Institute for Stem Cell Biology and Regenerative Medicine, Stanford University School of Medicine, Stanford, CA 94305.
  • Kuschner WG; Department of Bioengineering, University of California, Berkeley, CA 94720.
  • Schwartz EJ; Department of Radiology, Stanford University School of Medicine, Stanford, CA 94305.
  • Shrager JB; Department of Radiation Oncology, Stanford University School of Medicine, Stanford, CA 94305.
  • Neal JW; Department of Radiation Oncology, Stanford University School of Medicine, Stanford, CA 94305.
  • Wakelee HA; Department of Bioengineering, University of California, Berkeley, CA 94720.
  • Diehn M; Section of Pulmonary and Critical Care Medicine, Veterans Affairs Palo Alto Health Care System, Palo Alto, CA 94304.
  • Nair VS; Division of Pulmonary and Critical Care Medicine, Department of Medicine, Stanford University School of Medicine, Stanford, CA 94305.
  • Wang SX; Department of Pathology, Stanford University School of Medicine, Stanford, CA 94305.
  • Gambhir SS; Department of Cardiothoracic Surgery, Stanford University School of Medicine, Stanford, CA 94305.
Proc Natl Acad Sci U S A ; 113(52): E8379-E8386, 2016 12 27.
Article em En | MEDLINE | ID: mdl-27956614
ABSTRACT
Circulating tumor cells (CTCs) are established cancer biomarkers for the "liquid biopsy" of tumors. Molecular analysis of single CTCs, which recapitulate primary and metastatic tumor biology, remains challenging because current platforms have limited throughput, are expensive, and are not easily translatable to the clinic. Here, we report a massively parallel, multigene-profiling nanoplatform to compartmentalize and analyze hundreds of single CTCs. After high-efficiency magnetic collection of CTC from blood, a single-cell nanowell array performs CTC mutation profiling using modular gene panels. Using this approach, we demonstrated multigene expression profiling of individual CTCs from non-small-cell lung cancer (NSCLC) patients with remarkable sensitivity. Thus, we report a high-throughput, multiplexed strategy for single-cell mutation profiling of individual lung cancer CTCs toward minimally invasive cancer therapy prediction and disease monitoring.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Biomarcadores Tumorais / Carcinoma Pulmonar de Células não Pequenas / Neoplasias Pulmonares / Células Neoplásicas Circulantes Limite: Adult / Aged / Female / Humans / Male / Middle aged Idioma: En Revista: Proc Natl Acad Sci U S A Ano de publicação: 2016 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Biomarcadores Tumorais / Carcinoma Pulmonar de Células não Pequenas / Neoplasias Pulmonares / Células Neoplásicas Circulantes Limite: Adult / Aged / Female / Humans / Male / Middle aged Idioma: En Revista: Proc Natl Acad Sci U S A Ano de publicação: 2016 Tipo de documento: Article