A comparison of nanoparticle-antibody conjugation strategies in sandwich immunoassays.
J Immunoassay Immunochem
; 38(4): 355-377, 2017.
Article
em En
| MEDLINE
| ID: mdl-27982728
ABSTRACT
Point-of-care (POC) diagnostics such as lateral flow and dipstick immunoassays use gold nanoparticle (NP)-antibody conjugates for visual readout. We investigated the effects of NP conjugation, surface chemistries, and antibody immobilization methods on dipstick performance. We compared orientational, covalent conjugation, electrostatic adsorption, and a commercial conjugation kit for dipstick assays to detect dengue virus NS1 protein. Assay performance depended significantly on their conjugate properties. We also tested arrangements of multiple test lines within strips. Results show that orientational, covalent conjugation with PEG shield could improve NS1 detection. These approaches can be used to optimize immunochromatographic detection for a range of biomarkers.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Imunoensaio
/
Proteínas não Estruturais Virais
/
Nanopartículas Metálicas
/
Anticorpos
Limite:
Humans
Idioma:
En
Revista:
J Immunoassay Immunochem
Assunto da revista:
ALERGIA E IMUNOLOGIA
Ano de publicação:
2017
Tipo de documento:
Article