An oxygen sensitive self-decision making engineered CAR T-cell.
Sci Rep
; 7: 39833, 2017 01 20.
Article
em En
| MEDLINE
| ID: mdl-28106050
ABSTRACT
A key to the success of chimeric antigen receptor (CAR) T-cell based therapies greatly rely on the capacity to identify and target antigens with expression restrained to tumor cells. Here we present a strategy to generate CAR T-cells that are only effective locally (tumor tissue), potentially also increasing the choice of targetable antigens. By fusing an oxygen sensitive subdomain of HIF1α to a CAR scaffold, we generated CAR T-cells that are responsive to a hypoxic environment, a hallmark of certain tumors. Along with the development of oxygen-sensitive CAR T-cells, this work also provides a basic framework to use a multi-chain CAR as a platform to create the next generation of smarter self-decision making CAR T-cells.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Oxigênio
/
Proteínas Recombinantes de Fusão
/
Receptores de Antígenos de Linfócitos T
/
Linfócitos T Citotóxicos
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Imunoterapia Adotiva
/
Subunidade alfa do Fator 1 Induzível por Hipóxia
/
Neoplasias
Tipo de estudo:
Diagnostic_studies
/
Prognostic_studies
Limite:
Humans
Idioma:
En
Revista:
Sci Rep
Ano de publicação:
2017
Tipo de documento:
Article
País de afiliação:
Estados Unidos