Validation of a monoclonal antibody-based immunofluorescent assay to detect Burkholderia pseudomallei in blood cultures.
Trans R Soc Trop Med Hyg
; 110(11): 670-672, 2016 Nov 01.
Article
em En
| MEDLINE
| ID: mdl-28115683
ABSTRACT
BACKGROUND:
Identification of Burkholderia pseudomallei, the cause of melioidosis, using routine methods takes several days. Use of a monoclonal antibody-based immunofluorescent assay (IFA) on positive blood cultures may speed diagnosis.METHODS:
We tested the diagnostic accuracy of the IFA on 545 blood cultures positive for Gram-negative organisms at Udon Thani Hospital, Thailand, between June 2015 and August 2016.RESULTS:
Sensitivity of the IFA was 100% and specificity was 99.6%. The median decrease in time to pathogen identification between the IFA result and routine methods was 28 h (IQR 25-51), p<0.0001.CONCLUSIONS:
The IFA accurately expedites the diagnosis of melioidosis.Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Burkholderia pseudomallei
/
Melioidose
Tipo de estudo:
Diagnostic_studies
/
Prognostic_studies
Limite:
Humans
Idioma:
En
Revista:
Trans R Soc Trop Med Hyg
Ano de publicação:
2016
Tipo de documento:
Article
País de afiliação:
Tailândia