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Effects of acidic pH on voltage-gated ion channels in rat trigeminal mesencephalic nucleus neurons.
Han, Jin-Eon; Cho, Jin-Hwa; Choi, In-Sun; Kim, Do-Yeon; Jang, Il-Sung.
Afiliação
  • Han JE; Department of Pharmacology, School of Dentistry, Kyungpook National University, Daegu 41940, Korea.
  • Cho JH; Department of Pharmacology, School of Dentistry, Kyungpook National University, Daegu 41940, Korea.
  • Choi IS; Department of Pharmacology, School of Dentistry, Kyungpook National University, Daegu 41940, Korea.
  • Kim DY; Department of Pharmacology, School of Dentistry, Kyungpook National University, Daegu 41940, Korea.; Brain Science & Engineering Institute, Kyungpook National University, Daegu 41940, Korea.
  • Jang IS; Department of Pharmacology, School of Dentistry, Kyungpook National University, Daegu 41940, Korea.; Brain Science & Engineering Institute, Kyungpook National University, Daegu 41940, Korea.
Korean J Physiol Pharmacol ; 21(2): 215-223, 2017 Mar.
Article em En | MEDLINE | ID: mdl-28280415
ABSTRACT
The effects of acidic pH on several voltage-dependent ion channels, such as voltage-dependent K+ and Ca2+ channels, and hyperpolarization-gated and cyclic nucleotide-activated cation (HCN) channels, were examined using a whole-cell patch clamp technique on mechanically isolated rat mesencephalic trigeminal nucleus neurons. The application of a pH 6.5 solution had no effect on the peak amplitude of voltage-dependent K+ currents. A pH 6.0 solution slightly, but significantly inhibited the peak amplitude of voltage-dependent K+ currents. The pH 6.0 also shifted both the current-voltage and conductance-voltage relationships to the depolarization range. The application of a pH 6.5 solution scarcely affected the peak amplitude of membrane currents mediated by HCN channels, which were profoundly inhibited by the general HCN channel blocker Cs+ (1 mM). However, the pH 6.0 solution slightly, but significantly inhibited the peak amplitude of HCN-mediated currents. Although the pH 6.0 solution showed complex modulation of the current-voltage and conductance-voltage relationships, the midpoint voltages for the activation of HCN channels were not changed by acidic pH. On the other hand, voltage-dependent Ca2+ channels were significantly inhibited by an acidic pH. The application of an acidic pH solution significantly shifted the current-voltage and conductance-voltage relationships to the depolarization range. The modulation of several voltage-dependent ion channels by an acidic pH might affect the excitability of mesencephalic trigeminal nucleus neurons, and thus physiological functions mediated by the mesencephalic trigeminal nucleus could be affected in acidic pH conditions.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Idioma: En Revista: Korean J Physiol Pharmacol Ano de publicação: 2017 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Idioma: En Revista: Korean J Physiol Pharmacol Ano de publicação: 2017 Tipo de documento: Article