Analysis of sequential changes in major histocompatibility complex expression in human liver grafts after transplantation.
Transplantation
; 45(2): 394-401, 1988 Feb.
Article
em En
| MEDLINE
| ID: mdl-2830687
ABSTRACT
The expression of class I and class II major histocompatibility complex (MHC) antigens in human liver grafts was analyzed in 88 liver biopsies from 22 patients. For the staining of MHC antigens, a panel of monoclonal antibodies directed against monomorphic determinants of class I and class II molecules and an indirect immunoperoxidase method were used. In the reference biopsies, class I antigens were expressed on all cell types but only weakly on hepatocytes; class II was only expressed on Kupffer cells, interstitial cells, and endothelia. After transplantation, this pattern of MHC expression was markedly modified. Increased class I expression on hepatocytes and HLA-DR expression on bile ducts occurred in the absence of clinical rejection. During acute rejection, class I was strongly expressed and HLA-DR weakly expressed on hepatocytes; on bile ducts, HLA-DR expression was further increased. Cytomegalovirus hepatitis caused class I and HLA-DR induction on hepatocytes; strong induction of HLA-DR on bile ducts was also found in cholangitis. These findings have a number of implications for the pathophysiology of rejection of the transplanted liver.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Antígenos de Histocompatibilidade Classe II
/
Transplante de Fígado
/
Antígenos HLA
Tipo de estudo:
Etiology_studies
/
Observational_studies
/
Prognostic_studies
/
Risk_factors_studies
Limite:
Adult
/
Female
/
Humans
/
Male
/
Middle aged
Idioma:
En
Revista:
Transplantation
Ano de publicação:
1988
Tipo de documento:
Article