Transcriptome-Wide Detection of 5-Methylcytosine by Bisulfite Sequencing.
Methods Mol Biol
; 1562: 123-142, 2017.
Article
em En
| MEDLINE
| ID: mdl-28349458
ABSTRACT
While low-throughput RNA bisulfite sequencing is the method of choice to assess the methylation status of specific cytosines in candidate RNAs, the combination of bisulfite treatment of RNA with today's high-throughput sequencing techniques opens the door to methylation studies at nucleotide resolution on a transcriptome-wide scale. Below we describe a protocol for the transcriptome-wide analysis of total or fractionated poly(A)RNA in cells and tissues. Although the nature of the bisulfite sequencing protocol makes it comparably easy to translate from a low to a high-throughput approach, several critical points require attention before starting such a project. We describe a step-by-step protocol for planning and performing the experiment and analyzing the data.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
RNA
/
5-Metilcitosina
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Sequenciamento de Nucleotídeos em Larga Escala
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Transcriptoma
Tipo de estudo:
Diagnostic_studies
/
Guideline
Idioma:
En
Revista:
Methods Mol Biol
Assunto da revista:
BIOLOGIA MOLECULAR
Ano de publicação:
2017
Tipo de documento:
Article
País de afiliação:
Áustria