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Molecular MR Imaging of Myeloperoxidase Distinguishes Steatosis from Steatohepatitis in Nonalcoholic Fatty Liver Disease.
Pulli, Benjamin; Wojtkiewicz, Gregory; Iwamoto, Yoshiko; Ali, Muhammad; Zeller, Matthias W; Bure, Lionel; Wang, Cuihua; Choi, Yuri; Masia, Ricard; Guimaraes, Alex R; Corey, Kathleen E; Chen, John W.
Afiliação
  • Pulli B; From the Center for Systems Biology (B.P., G.W., Y.I., M.A., M.W.Z., L.B., C.W., A.R.G., J.W.C.), Department of Radiology (B.P., A.R.G., J.W.C.), Liver Center and Gastrointestinal Division (Y.C., K.E.C.), and Department of Pathology (R.M.), Massachusetts General Hospital and Harvard Medical School,
  • Wojtkiewicz G; From the Center for Systems Biology (B.P., G.W., Y.I., M.A., M.W.Z., L.B., C.W., A.R.G., J.W.C.), Department of Radiology (B.P., A.R.G., J.W.C.), Liver Center and Gastrointestinal Division (Y.C., K.E.C.), and Department of Pathology (R.M.), Massachusetts General Hospital and Harvard Medical School,
  • Iwamoto Y; From the Center for Systems Biology (B.P., G.W., Y.I., M.A., M.W.Z., L.B., C.W., A.R.G., J.W.C.), Department of Radiology (B.P., A.R.G., J.W.C.), Liver Center and Gastrointestinal Division (Y.C., K.E.C.), and Department of Pathology (R.M.), Massachusetts General Hospital and Harvard Medical School,
  • Ali M; From the Center for Systems Biology (B.P., G.W., Y.I., M.A., M.W.Z., L.B., C.W., A.R.G., J.W.C.), Department of Radiology (B.P., A.R.G., J.W.C.), Liver Center and Gastrointestinal Division (Y.C., K.E.C.), and Department of Pathology (R.M.), Massachusetts General Hospital and Harvard Medical School,
  • Zeller MW; From the Center for Systems Biology (B.P., G.W., Y.I., M.A., M.W.Z., L.B., C.W., A.R.G., J.W.C.), Department of Radiology (B.P., A.R.G., J.W.C.), Liver Center and Gastrointestinal Division (Y.C., K.E.C.), and Department of Pathology (R.M.), Massachusetts General Hospital and Harvard Medical School,
  • Bure L; From the Center for Systems Biology (B.P., G.W., Y.I., M.A., M.W.Z., L.B., C.W., A.R.G., J.W.C.), Department of Radiology (B.P., A.R.G., J.W.C.), Liver Center and Gastrointestinal Division (Y.C., K.E.C.), and Department of Pathology (R.M.), Massachusetts General Hospital and Harvard Medical School,
  • Wang C; From the Center for Systems Biology (B.P., G.W., Y.I., M.A., M.W.Z., L.B., C.W., A.R.G., J.W.C.), Department of Radiology (B.P., A.R.G., J.W.C.), Liver Center and Gastrointestinal Division (Y.C., K.E.C.), and Department of Pathology (R.M.), Massachusetts General Hospital and Harvard Medical School,
  • Choi Y; From the Center for Systems Biology (B.P., G.W., Y.I., M.A., M.W.Z., L.B., C.W., A.R.G., J.W.C.), Department of Radiology (B.P., A.R.G., J.W.C.), Liver Center and Gastrointestinal Division (Y.C., K.E.C.), and Department of Pathology (R.M.), Massachusetts General Hospital and Harvard Medical School,
  • Masia R; From the Center for Systems Biology (B.P., G.W., Y.I., M.A., M.W.Z., L.B., C.W., A.R.G., J.W.C.), Department of Radiology (B.P., A.R.G., J.W.C.), Liver Center and Gastrointestinal Division (Y.C., K.E.C.), and Department of Pathology (R.M.), Massachusetts General Hospital and Harvard Medical School,
  • Guimaraes AR; From the Center for Systems Biology (B.P., G.W., Y.I., M.A., M.W.Z., L.B., C.W., A.R.G., J.W.C.), Department of Radiology (B.P., A.R.G., J.W.C.), Liver Center and Gastrointestinal Division (Y.C., K.E.C.), and Department of Pathology (R.M.), Massachusetts General Hospital and Harvard Medical School,
  • Corey KE; From the Center for Systems Biology (B.P., G.W., Y.I., M.A., M.W.Z., L.B., C.W., A.R.G., J.W.C.), Department of Radiology (B.P., A.R.G., J.W.C.), Liver Center and Gastrointestinal Division (Y.C., K.E.C.), and Department of Pathology (R.M.), Massachusetts General Hospital and Harvard Medical School,
  • Chen JW; From the Center for Systems Biology (B.P., G.W., Y.I., M.A., M.W.Z., L.B., C.W., A.R.G., J.W.C.), Department of Radiology (B.P., A.R.G., J.W.C.), Liver Center and Gastrointestinal Division (Y.C., K.E.C.), and Department of Pathology (R.M.), Massachusetts General Hospital and Harvard Medical School,
Radiology ; 284(2): 390-400, 2017 08.
Article em En | MEDLINE | ID: mdl-28358240
ABSTRACT
Purpose To test whether MPO-Gd, an activatable molecular magnetic resonance (MR) imaging agent specific for myeloperoxidase (MPO) activity, could detect MPO activity in nonalcoholic steatohepatitis (NASH) mouse models and human liver biopsy samples. Materials and Methods In this study, 20 leptin receptor-deficient and three MPO knockout mice were injected with endotoxin (lipopolysaccharide) or fed a methionine and choline-deficient (MCD) diet to induce experimental NASH and underwent MR imaging with MPO-Gd. Saline-injected and control diet-fed leptin receptor-deficient mice were used as respective controls. MPO protein and activity measurements and histologic analyses were performed. Eleven human liver biopsy samples underwent MPO-Gd-enhanced MR imaging ex vivo and subsequent histologic evaluation. Results were compared with Student t test or Mann-Whitney U test. Results With endotoxin, a significantly increased contrast-to-noise ratio (CNR) was found compared with sham (mean CNR, 1.81 [95% confidence interval {CI} 1.53, 2.10] vs 1.02 [95% CI 0.89, 1.14]; P = .03) at MPO-Gd MR imaging. In the diet-induced NASH model, an increased CNR was also found compared with sham mice (mean CNR, 1.33 [95% CI 1.27, 1.40] vs 0.98 [95% CI 0.83, 1.12]; P = .008). Conversely, CNR remained at baseline in NASH mice imaged with gadopentetate dimeglumine and in MPO knockout NASH mice with MPO-Gd, which proves specificity of MPO-Gd. Ex vivo molecular MR imaging of liver biopsy samples from NASH and control patients confirmed results from animal studies (mean CNR for NASH vs control patients, 2.61 [95% CI 1.48, 3.74] vs 1.29 [95% CI 1.06, 1.52]; P = .004). Conclusion MPO-Gd showed elevated MPO activity in NAFLD mouse models and human liver biopsy samples. © RSNA, 2017 Online supplemental material is available for this article. An earlier incorrect version of this article appeared online. This article was corrected on April 6, 2017.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Imageamento por Ressonância Magnética / Peroxidase / Fígado Gorduroso / Imagem Molecular / Hepatopatia Gordurosa não Alcoólica Tipo de estudo: Diagnostic_studies Limite: Adult / Animals / Female / Humans / Middle aged Idioma: En Revista: Radiology Ano de publicação: 2017 Tipo de documento: Article
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Imageamento por Ressonância Magnética / Peroxidase / Fígado Gorduroso / Imagem Molecular / Hepatopatia Gordurosa não Alcoólica Tipo de estudo: Diagnostic_studies Limite: Adult / Animals / Female / Humans / Middle aged Idioma: En Revista: Radiology Ano de publicação: 2017 Tipo de documento: Article