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SHORT-COMMUNICATION Evaluation of perfused bovine udder for gene expression studies in dairy cows.
Pinto, I S B; Fonseca, I; Brandão, H M; Gern, J C; Guimarães, A S; Carvalho, W A; Brito, M A V P; Viccini, L F; Martins, M F.
Afiliação
  • Pinto IS; Universidade Federal de Juiz de Fora, Departamento de Biologia, Instituto de Ciências Biológicas, Juiz de Fora, MG, Brasil.
  • Fonseca I; Instituto Federal Catarinense, Campus Videira, Videira, SC, Brasil.
  • Brandão HM; Embrapa Gado de Leite, Juiz de Fora, MG, Brasil.
  • Gern JC; Embrapa Gado de Leite, Juiz de Fora, MG, Brasil.
  • Guimarães AS; Embrapa Gado de Leite, Juiz de Fora, MG, Brasil.
  • Carvalho WA; Embrapa Gado de Leite, Juiz de Fora, MG, Brasil.
  • Brito MA; Embrapa Gado de Leite, Juiz de Fora, MG, Brasil.
  • Viccini LF; Universidade Federal de Juiz de Fora, Departamento de Biologia, Instituto de Ciências Biológicas, Juiz de Fora, MG, Brasil.
  • Martins MF; Embrapa Gado de Leite, Juiz de Fora, MG, Brasil marta.martins@embrapa.br.
Genet Mol Res ; 16(1)2017 Mar 30.
Article em En | MEDLINE | ID: mdl-28363011
Intramammary infections are one of the main causes of productivity loss in dairy cows. To better understand the immune system response and to avoid the use of live animals, we validated the use of isolated bovine udder as an ex situ model. Six mammary glands were collected from cows ready for culling. Three udders were perfused with Tyrode's solution and three were not-perfused. During six hours, we collected perfusate samples for biochemical analysis. We also collected alveolar and teat canal tissue to evaluate gene expression. The biochemical parameters indicated that the perfused udders remained viable for the entire period of the experiment. A real-time polymerase chain reaction showed an increase in 18S rRNA gene expression in the alveolar tissue at 3 and 4 h after perfusion. There was also an increase in the Ubiquitin gene in the teat canal from not-perfused udders at 1, 3, and 4 h after slaughter. In general, gene expression was stable during the experiment. Our results indicated that the isolated perfused bovine udder model is appropriate for genetic studies, opening a new perspective in animal experimentation methods.
Assuntos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Glândulas Mamárias Animais Tipo de estudo: Prognostic_studies Limite: Animals Idioma: En Revista: Genet Mol Res Assunto da revista: BIOLOGIA MOLECULAR / GENETICA Ano de publicação: 2017 Tipo de documento: Article País de afiliação: Brasil

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Glândulas Mamárias Animais Tipo de estudo: Prognostic_studies Limite: Animals Idioma: En Revista: Genet Mol Res Assunto da revista: BIOLOGIA MOLECULAR / GENETICA Ano de publicação: 2017 Tipo de documento: Article País de afiliação: Brasil