Identification and expression analysis of terminal deoxynucleotidyl transferase in humphead snapper Lutjanus sanguineus.

Huang, Y; Cai, J; Tang, J F; Zhang, H Y; Wang, Z W; Jian, J C; Wu, Z H; Lu, Y S

Huang, Y; Cai, J; Tang, J F; Zhang, H Y; Wang, Z W; Jian, J C; Wu, Z H; Lu, Y S.

Afiliação

Huang Y; College of Fishery, Guangdong Ocean University, Zhanjiang, 524088, People's Republic of China.
Cai J; Guangdong Provincial Key Laboratory of Pathogenic Biology and Epidemiology for Aquatic Economic Animals, Zhanjiang, 524088, People's Republic of China.
Tang JF; Guangdong Key Laboratory of Control for Diseases of Aquatic Economic Animals, Zhanjiang, 524088, People's Republic of China.
Zhang HY; College of Fishery, Guangdong Ocean University, Zhanjiang, 524088, People's Republic of China.
Wang ZW; Guangdong Provincial Key Laboratory of Pathogenic Biology and Epidemiology for Aquatic Economic Animals, Zhanjiang, 524088, People's Republic of China.
Jian JC; Guangdong Key Laboratory of Control for Diseases of Aquatic Economic Animals, Zhanjiang, 524088, People's Republic of China.
Wu ZH; College of Fishery, Guangdong Ocean University, Zhanjiang, 524088, People's Republic of China.
Lu YS; Guangdong Provincial Key Laboratory of Pathogenic Biology and Epidemiology for Aquatic Economic Animals, Zhanjiang, 524088, People's Republic of China.

J Fish Biol ; 90(5): 2194-2199, 2017 May.

Article em En | MEDLINE | ID: mdl-28369937

ABSTRACT

ABSTRACT

A tdt gene was identified successfully from humphead snapper Lutjanus sanguineus, which contained 1710 bp encoding a protein of 463 amino acids. Results of quantitative real-time polymerase chain reaction (qRT-PCR) indicated that tdt mainly expressed in thymus and head kidney and the transcripts of tdt in these tissues were up-regulated significantly at 36 and 48 h after Vibrio harveyi infection. Meanwhile Tdt-producing cells were found in thymus and head kidney.

Assuntos

DNA Nucleotidilexotransferase/metabolismo; Proteínas de Peixes/metabolismo; Regulação Enzimológica da Expressão Gênica/fisiologia; Perciformes/metabolismo; Sequência de Aminoácidos; Animais; DNA Nucleotidilexotransferase/genética; Proteínas de Peixes/genética; Perciformes/genética; Filogenia; Reação em Cadeia da Polimerase em Tempo Real

Palavras-chave

TdT; Vibrio harveyi; immunohistochemistry; tdt gene

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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Perciformes / Regulação Enzimológica da Expressão Gênica / Proteínas de Peixes / DNA Nucleotidilexotransferase Tipo de estudo: Diagnostic_studies Limite: Animals Idioma: En Revista: J Fish Biol Ano de publicação: 2017 Tipo de documento: Article

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