Validated method for the determination of propane-1,2-diol, butane-2,3-diol, and propane-1,3-diol in cheese and bacterial cultures using phenylboronic esterification and GC-MS.
Food Chem
; 230: 372-377, 2017 Sep 01.
Article
em En
| MEDLINE
| ID: mdl-28407924
ABSTRACT
A simple, fast, sensitive, and robust gas chromatography-mass spectrometry (GC-MS) method for the simultaneous determination of propane-1,2-diol, butane-2,3-diol, and propane-1,3-diol in cheese and bacterial cultures was developed. Target analytes were extracted and transformed into their phenylboronic esters prior to analysis. The method showed good sensitivity, without carryover between the samples. The detection limits for propane-1,2-diol, butane-2,3-diol, and propane-1,3-diol in cheese samples were 0.26, 0.02, and 0.11mgkg-1, respectively, and for bacterial culture samples were 1.32, 0.09, and 0.54mgkg-1, respectively. The Horwitz ratio showed good precision for all analytes (<0.45). The calibrated range in cheese for all analytes was very broad, from 0 to 1000mgkg-1, and in bacterial cultures was from 0 to 5000mgkg-1 with R2>0.9991. The results confirm excellent applicability of the proposed method for the determination of the target metabolites in cheese and bacterial culture samples.
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Texto completo:
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Propano
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Ácidos Borônicos
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Butanos
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Queijo
/
Ésteres
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Cromatografia Gasosa-Espectrometria de Massas
Idioma:
En
Revista:
Food Chem
Ano de publicação:
2017
Tipo de documento:
Article