Three new active members of the I-OnuI family of homing endonucleases.
Can J Microbiol
; 63(8): 671-681, 2017 Aug.
Article
em En
| MEDLINE
| ID: mdl-28414922
ABSTRACT
In vitro characterization of 3 LAGLIDADG-type homing endonucleases (HEs) (I-CcaI, I-CcaII, and I-AstI) that belong to the I-OnuI family showed that they are functional HEs that cleave their respective cognate target sites. These endonucleases are encoded within group ID introns and appear to be orthologues that have inserted into 3 different mitochondrial genes rns, rnl, and cox3. The endonuclease activity of I-CcaI was tested using various substrates, and its minimum DNA recognition sequence was estimated to be 26 nt. This set of HEs may provide some insight into how these types of mobile elements can migrate into new locations. This study provides additional endonucleases that can be added to the catalog of currently available HEs that may have various biotechnology applications.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Endonucleases
Idioma:
En
Revista:
Can J Microbiol
Ano de publicação:
2017
Tipo de documento:
Article
País de afiliação:
Canadá