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Human Epicardial Fat Expresses Glucagon-Like Peptide 1 and 2 Receptors Genes.
Iacobellis, Gianluca; Camarena, Vladimir; Sant, David W; Wang, Gaofeng.
Afiliação
  • Iacobellis G; Division of Endocrinology, Diabetes and Metabolism, Department of Medicine, University of Miami Miller School of Medicine, Miami, USA.
  • Camarena V; John P. Hussman Institute for Human Genomics, Dr. John T. Macdonald Foundation Department of Human Genetics, University of Miami Miller School of Medicine, Miami, USA.
  • Sant DW; John P. Hussman Institute for Human Genomics, Dr. John T. Macdonald Foundation Department of Human Genetics, University of Miami Miller School of Medicine, Miami, USA.
  • Wang G; John P. Hussman Institute for Human Genomics, Dr. John T. Macdonald Foundation Department of Human Genetics, University of Miami Miller School of Medicine, Miami, USA.
Horm Metab Res ; 49(8): 625-630, 2017 Aug.
Article em En | MEDLINE | ID: mdl-28514806
ABSTRACT
Epicardial adipose tissue (EAT) is an easily measurable visceral fat of the heart with unique anatomy, functionality, and transcriptome. EAT can serve as a therapeutic target for pharmaceutical agents targeting the fat. Glucagon-like peptide-1 (GLP-1) and GLP-2 analogues are newer drugs showing beneficial cardiovascular and metabolic effects. Whether EAT expresses GLP- 1 and 2 receptors (GLP-1R and GLP-2R) is unknown. RNA-seq analysis and quantitative real-time polymerase chain reaction (qRT-PCR) were performed to evaluate the presence of GLP-1R and GLP-2R in EAT and subcutaneous fat (SAT) obtained from 8 subjects with coronary artery disease and type 2 diabetes mellitus undergoing elective cardiac surgery. Immunofluorescence was also performed on EAT and SAT samples using Mab3f52 against GLP-1R. Our RNA-sequencing (RNA-seq) analysis showed that EAT expresses both GLP-1R and GLP-2R genes. qRT-PCR analysis confirmed that GLP-1R expression was low but detected by 2 different sets of intron-spanning primers. GLP-2R expression was detected in all patients and was found to be 5-fold higher than GLP-1R. The combination of accurately spliced reads from RNA-seq and successful amplification using intron-spanning primers indicates that both GLP-1R and GLP-2R are expressed in EAT. Immunofluorescence clearly showed that GLP-1R is present and more abundant in EAT than SAT. This is the first time that human EAT is found to express both GLP-1R and GLP-2R genes. Pharmacologically targeting EAT may induce beneficial cardiovascular and metabolic effects.
Assuntos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Pericárdio / Tecido Adiposo / Regulação da Expressão Gênica / Diabetes Mellitus Tipo 2 / Receptor do Peptídeo Semelhante ao Glucagon 1 / Receptor do Peptídeo Semelhante ao Glucagon 2 Limite: Female / Humans / Male Idioma: En Revista: Horm Metab Res Ano de publicação: 2017 Tipo de documento: Article País de afiliação: Estados Unidos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Pericárdio / Tecido Adiposo / Regulação da Expressão Gênica / Diabetes Mellitus Tipo 2 / Receptor do Peptídeo Semelhante ao Glucagon 1 / Receptor do Peptídeo Semelhante ao Glucagon 2 Limite: Female / Humans / Male Idioma: En Revista: Horm Metab Res Ano de publicação: 2017 Tipo de documento: Article País de afiliação: Estados Unidos