Contryphan-Bt: A pyroglutamic acid containing conopeptide isolated from the venom of Conus betulinus.
Toxicon
; 135: 17-23, 2017 Sep 01.
Article
em En
| MEDLINE
| ID: mdl-28554718
ABSTRACT
A new member of the contryphans family was isolated from the venom of Conus betilinus, a vermivorous species distributed in the South China Sea. Its sequence, ZSGCO(D-W)KPWC-NH2 (Z, pyroglutamic acid), was established by a combination of de novo MS/MS sequencing and venom-duct transcriptome sequencing. The occurrence of D-Trp6 was confirmed by chemical synthesis and HPLC behavior comparison. Like known contryphans, contryphan-Bt produces the "stiff-tail" syndrome in mice and contains one disulfide bond, a hydroxyproline, a D-tryptophan, and an amidated C-terminus. However, contryphan-Bt differs from previously identified contryphans by a pyroglutamic acid at the N terminus. CD spectrum reveals that contryphan-Bt possess ß-turn in solution.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Peptídeos Cíclicos
/
Caramujo Conus
Limite:
Animals
Idioma:
En
Revista:
Toxicon
Ano de publicação:
2017
Tipo de documento:
Article
País de afiliação:
China