Your browser doesn't support javascript.
loading
Characterization of the rcsA Gene from Pantoea sp. Strain PPE7 and Its Influence on Extracellular Polysaccharide Production and Virulence on Pleurotus eryngii.
Kim, Min Keun; Lee, Sun Mi; Seuk, Su Won; Ryu, Jae San; Kim, Hee Dae; Kwon, Jin Hyeuk; Choi, Yong Jo; Yun, Han Dae.
Afiliação
  • Kim MK; Environment-Friendly Research Division, Gyeongsangnam-do Agricultural Research & Extension Services, Jinju 52733, Korea.
  • Lee SM; Environment-Friendly Research Division, Gyeongsangnam-do Agricultural Research & Extension Services, Jinju 52733, Korea.
  • Seuk SW; Environment-Friendly Research Division, Gyeongsangnam-do Agricultural Research & Extension Services, Jinju 52733, Korea.
  • Ryu JS; Environment-Friendly Research Division, Gyeongsangnam-do Agricultural Research & Extension Services, Jinju 52733, Korea.
  • Kim HD; Environment-Friendly Research Division, Gyeongsangnam-do Agricultural Research & Extension Services, Jinju 52733, Korea.
  • Kwon JH; Environment-Friendly Research Division, Gyeongsangnam-do Agricultural Research & Extension Services, Jinju 52733, Korea.
  • Choi YJ; Environment-Friendly Research Division, Gyeongsangnam-do Agricultural Research & Extension Services, Jinju 52733, Korea.
  • Yun HD; Department of Applied Life Chemistry, College of Agriculture and Life Science, Gyeongsang National University, JinJu 52828, Korea.
Plant Pathol J ; 33(3): 276-287, 2017 Jun.
Article em En | MEDLINE | ID: mdl-28592946
RcsA is a positive activator of extracellular polysaccharide (EPS) synthesis in the Enterobacteriaceae. The rcsA gene of the soft rot pathogen Pantoea sp. strain PPE7 in Pleurotus eryngii was cloned by PCR amplification, and its role in EPS synthesis and virulence was investigated. The RcsA protein contains 3 highly conserved domains, and the C-terminal end of the open reading frame shared significant amino acid homology to the helix-turn-helix DNA binding motif of bacterial activator proteins. The inactivation of rcsA by insertional mutagenesis created mutants that had decreased production of EPS compared to the wild-type strain and abolished the virulence of Pantoea sp. strain PPE7 in P. eryngii. The Pantoea sp. strain PPE7 rcsA gene was shown to strongly affect the formation of the disease symptoms of a mushroom pathogen and to act as the virulence factor to cause soft rot disease in P. eryngii.
Palavras-chave

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Idioma: En Revista: Plant Pathol J Ano de publicação: 2017 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Idioma: En Revista: Plant Pathol J Ano de publicação: 2017 Tipo de documento: Article