Cell-Free Synthetic Biology Chassis for Nanocatalytic Photon-to-Hydrogen Conversion.
ACS Nano
; 11(7): 6739-6745, 2017 07 25.
Article
em En
| MEDLINE
| ID: mdl-28602073
ABSTRACT
We report on an entirely man-made nano-bio architecture fabricated through noncovalent assembly of a cell-free expressed transmembrane proton pump and TiO2 semiconductor nanoparticles as an efficient nanophotocatalyst for H2 evolution. The system produces hydrogen at a turnover of about 240 µmol of H2 (µmol protein)-1 h-1 and 17.74 mmol of H2 (µmol protein)-1 h-1 under monochromatic green and white light, respectively, at ambient conditions, in water at neutral pH and room temperature, with methanol as a sacrificial electron donor. Robustness and flexibility of this approach allow for systemic manipulation at the nanoparticle-bio interface toward directed evolution of energy transformation materials and artificial systems.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Titânio
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Bacteriorodopsinas
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Fótons
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Halobacterium salinarum
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Pontos Quânticos
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Proteínas Imobilizadas
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Hidrogênio
Idioma:
En
Revista:
ACS Nano
Ano de publicação:
2017
Tipo de documento:
Article
País de afiliação:
Estados Unidos