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Angiostrongylus cantonensis: An optimized cultivation of this parasitic nematode under laboratory conditions.
Xie, Hui; Yuan, Dongjuan; Luo, Shiqi; Zeng, Xingda; Zeng, Xin; He, Ping; Lv, Zhiyue; Wu, Zhongdao.
Afiliação
  • Xie H; Department of Parasitology, Zhongshan School of Medicine, Sun Yat-sen University, No. 74, Zhongshan Rd. 2, Guangzhou, 510080, People's Republic of China.
  • Yuan D; Key Laboratory for Tropical Diseases Control (Sun Yat-sen University), The National Ministry of Education, Guangzhou, 510080, People's Republic of China.
  • Luo S; Department of Parasitology, Zhongshan School of Medicine, Sun Yat-sen University, No. 74, Zhongshan Rd. 2, Guangzhou, 510080, People's Republic of China. dongjuanyuan@foxmail.com.
  • Zeng X; Key Laboratory for Tropical Diseases Control (Sun Yat-sen University), The National Ministry of Education, Guangzhou, 510080, People's Republic of China. dongjuanyuan@foxmail.com.
  • Zeng X; Provincial Engineering Technology Research Center for Diseases-Vectors Control, Guangzhou, 510080, Guangdong, People's Republic of China. dongjuanyuan@foxmail.com.
  • He P; Department of Parasitology, Zhongshan School of Medicine, Sun Yat-sen University, No. 74, Zhongshan Rd. 2, Guangzhou, 510080, People's Republic of China.
  • Lv Z; Key Laboratory for Tropical Diseases Control (Sun Yat-sen University), The National Ministry of Education, Guangzhou, 510080, People's Republic of China.
  • Wu Z; Department of Parasitology, Zhongshan School of Medicine, Sun Yat-sen University, No. 74, Zhongshan Rd. 2, Guangzhou, 510080, People's Republic of China.
Parasitol Res ; 116(8): 2231-2237, 2017 Aug.
Article em En | MEDLINE | ID: mdl-28616635
ABSTRACT
Angiostrongylus cantonensis (A. cantonensis), a parasitic nematode, is the important neurotropic pathogen which causes human angiostrongyliasis. It has a complex life-cycle and severe parasite-host interaction in contrast to free-living nematode. Establishment of a well-suited life-cycle and in vitro cultivation of A. cantonensis in the laboratory will be one of the key techniques to elucidate the mechanism of parasite-host interaction. However, the low survival and growth rate of worms is still to be the problem. We optimized the known life-cycle of A. cantonensis in the laboratory, showing that small in size, easy to breed, and high compatibility of Biomphalaria straminea precede the common snails as an intermediate host of A. cantonensis. Furthermore, the egg hatching rate in Ham's F-12 medium reached approximately 80% using the eggs of mature female adult worms. We also demonstrated that the survival of larvae could be sustained for more than 30 days by in vitro cultivation of L1 larvae in DMEM with mixed antibiotics (100 units/mL of penicillin G potassium, 50 µg/mL of streptomycin sulfate, and 0.5 µg/mL of amphotericin B) and L3, L4, and L5 larvae in Waymouth's medium with 20% fetal calf serum and mixed antibiotics. Infective L1 and L3 larvae kept high infective rate to the snail and rat after cultivation in these media, respectively. It will provide the basis for studying on genetic manipulations for functional genes, new drug screening, and the mechanism of parasite-host interaction of parasitic nematodes.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Angiostrongylus cantonensis Limite: Animals Idioma: En Revista: Parasitol Res Assunto da revista: PARASITOLOGIA Ano de publicação: 2017 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Angiostrongylus cantonensis Limite: Animals Idioma: En Revista: Parasitol Res Assunto da revista: PARASITOLOGIA Ano de publicação: 2017 Tipo de documento: Article