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The CEP19-RABL2 GTPase Complex Binds IFT-B to Initiate Intraflagellar Transport at the Ciliary Base.
Kanie, Tomoharu; Abbott, Keene Louis; Mooney, Nancie Ann; Plowey, Edward Douglas; Demeter, Janos; Jackson, Peter Kent.
Afiliação
  • Kanie T; Baxter Laboratory, Department of Microbiology & Immunology, Stanford University School of Medicine, Stanford, CA 94305, USA.
  • Abbott KL; Baxter Laboratory, Department of Microbiology & Immunology, Stanford University School of Medicine, Stanford, CA 94305, USA.
  • Mooney NA; Baxter Laboratory, Department of Microbiology & Immunology, Stanford University School of Medicine, Stanford, CA 94305, USA.
  • Plowey ED; Department of Pathology, Stanford University School of Medicine, Stanford, CA 94305, USA.
  • Demeter J; Baxter Laboratory, Department of Microbiology & Immunology, Stanford University School of Medicine, Stanford, CA 94305, USA.
  • Jackson PK; Baxter Laboratory, Department of Microbiology & Immunology, Stanford University School of Medicine, Stanford, CA 94305, USA; Department of Pathology, Stanford University School of Medicine, Stanford, CA 94305, USA. Electronic address: pjackson@stanford.edu.
Dev Cell ; 42(1): 22-36.e12, 2017 07 10.
Article em En | MEDLINE | ID: mdl-28625565
Highly conserved intraflagellar transport (IFT) protein complexes direct both the assembly of primary cilia and the trafficking of signaling molecules. IFT complexes initially accumulate at the base of the cilium and periodically enter the cilium, suggesting an as-yet-unidentified mechanism that triggers ciliary entry of IFT complexes. Using affinity-purification and mass spectrometry of interactors of the centrosomal and ciliopathy protein, CEP19, we identify CEP350, FOP, and the RABL2B GTPase as proteins organizing the first known mechanism directing ciliary entry of IFT complexes. We discover that CEP19 is recruited to the ciliary base by the centriolar CEP350/FOP complex and then specifically captures GTP-bound RABL2B, which is activated via its intrinsic nucleotide exchange. Activated RABL2B then captures and releases its single effector, the intraflagellar transport B holocomplex, from the large pool of pre-docked IFT-B complexes, and thus initiates ciliary entry of IFT.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Cílios / Proteínas de Ciclo Celular / Proteínas rab de Ligação ao GTP / Complexos Multiproteicos / Flagelos Tipo de estudo: Prognostic_studies Limite: Animals Idioma: En Revista: Dev Cell Assunto da revista: EMBRIOLOGIA Ano de publicação: 2017 Tipo de documento: Article País de afiliação: Estados Unidos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Cílios / Proteínas de Ciclo Celular / Proteínas rab de Ligação ao GTP / Complexos Multiproteicos / Flagelos Tipo de estudo: Prognostic_studies Limite: Animals Idioma: En Revista: Dev Cell Assunto da revista: EMBRIOLOGIA Ano de publicação: 2017 Tipo de documento: Article País de afiliação: Estados Unidos