Vector competence of Malaysian Aedes albopictus with and without Wolbachia to four dengue virus serotypes.
Trop Med Int Health
; 22(9): 1154-1165, 2017 09.
Article
em En
| MEDLINE
| ID: mdl-28653334
ABSTRACT
OBJECTIVE:
To determine the susceptibility status of Aedes albopictus with and without Wolbachia to the four dengue virus serotypes.METHODS:
Two newly colonised colonies of Ae. albopictus from the wild were used for the study. One colony was naturally infected with Wolbachia while in the other Wolbachia was removed by tetracycline treatment. Both colonies were orally infected with dengue virus-infected fresh blood meal. Dengue virus load was measured using quantitative RT-PCR at four-time intervals in the salivary glands, midguts and ovaries.RESULTS:
Wolbachia did not significantly affect Malaysian Ae. albopictus dengue infection or the dissemination rate for all four dengue virus serotypes. Malaysian Ae. albopictus had the highest replication kinetics for DENV-1 and the highest salivary gland and midgut infection rate for DENV-4.CONCLUSION:
Wolbachia, which naturally exists in Malaysian Ae. albopictus, does not significantly affect dengue virus replication. Malaysian Ae. albopictus is susceptible to dengue virus infections and capable of transmitting dengue virus, especially DENV-1 and DENV-4. Removal of Wolbachia from Malaysian Ae. albopictus would not reduce their susceptibility status.Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Replicação Viral
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Aedes
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Wolbachia
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Dengue
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Vírus da Dengue
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Sorogrupo
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Insetos Vetores
Limite:
Animals
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Humans
Idioma:
En
Revista:
Trop Med Int Health
Assunto da revista:
MEDICINA TROPICAL
/
SAUDE PUBLICA
Ano de publicação:
2017
Tipo de documento:
Article
País de afiliação:
Malásia