Gossypol induces cell death by activating apoptosis and autophagy in HT-29 cells.
Mol Med Rep
; 16(2): 2128-2132, 2017 Aug.
Article
em En
| MEDLINE
| ID: mdl-28656225
ABSTRACT
Gossypol is a polyphenolic, yellowish compound derived from cottonseed extract. The present study examined the effects of gossypol on the apoptosis and autophagy of HT29 cells. A Cell Counting Kit8 assay, Annexin VFITC, JC1 staining and western blotting were used to identify the viability of cells, stages of apoptosis and the expression levels of the signaling proteins. Gossypol promoted apoptosis and induced the loss of mitochondrial membrane potential. Further investigation of the apoptotic mechanism revealed that gossypol increased the ratio of Bcell lymphoma 2 (Bcl-2)-associated X protein/Bcl2 protein levels and upregulated the expression of caspase3. Gossypol also enhanced the activity of microtubuleassociated protein light chain 3 LC3II and Beclin1 and downregulated LC3I, in a dosedependent manner. Together, these finding suggested that gossypol may be a novel and potential antitumor agent.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Autofagia
/
Gossipol
/
Apoptose
Tipo de estudo:
Prognostic_studies
Limite:
Humans
Idioma:
En
Revista:
Mol Med Rep
Ano de publicação:
2017
Tipo de documento:
Article