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Ultrasound processing of liquid system(s) and its antimicrobial mechanism of action.
Spiteri, D; Chot-Plassot, C; Sclear, J; Karatzas, K A; Scerri, C; Valdramidis, V P.
Afiliação
  • Spiteri D; Department of Food Studies and Environmental Health, University of Malta, Msida, Malta.
  • Chot-Plassot C; Centre for Molecular Medicine and Biobanking, University of Malta, Msida, Malta.
  • Sclear J; Department of Food Studies and Environmental Health, University of Malta, Msida, Malta.
  • Karatzas KA; Department of Food Studies and Environmental Health, University of Malta, Msida, Malta.
  • Scerri C; Department of Food and Nutritional Science, University of Reading, Reading, UK.
  • Valdramidis VP; Centre for Molecular Medicine and Biobanking, University of Malta, Msida, Malta.
Lett Appl Microbiol ; 65(4): 313-318, 2017 Oct.
Article em En | MEDLINE | ID: mdl-28727911
ABSTRACT
Ultrasound creates cavitation phenomena, resulting in the formation of several free radicals, namely OH˙ and H˙, due to the breakdown of the H2 O molecule. These radicals affect the cellular integrity of the bacteria, causing the inactivation of several processes, and thus it is important to unravel the mechanism of action of this technology. This research looks into the application and mechanism of action of ultrasound technology as a means of disinfection by acoustic cavitation. Sterile water and synthetic waste water were inoculated with different mutants of Escherichia coli K12 strains containing deletions in genes affecting specific functional properties of E. coli. These were dnak soxR, soxS, oxyR, rpoS, gadA/gadB, gadC and yneL. Escherichia coli K-12 ΔoxyR appeared to be more resistant to the treatment together with gadW, gadX, gabT and gabD, whereas the mutant K-12 ΔdnaK was more sensitive with c. 2·5 log (CFU per ml) reduction in comparison to their isogenic wild-type E. coli K-12. This indicates that the dnaK gene participates in general stress response and more specifically to hyperosmotic stress. The other E. coli deleted genes tested (soxS, rpoS, gadB, gadC, yneL) did not appear to be involved in protection of microbial cells against ultrasound. SIGNIFICANCE AND IMPACT OF THE STUDY This study looks at the mechanism of action of ultrasound technology for the disinfection of wastewater. Different mutants with deleted genes were used to study the respective sensitivity or resistance to this treatment. This is essential to characterize changes at the molecular level, which might be occurring during treatment, resulting in bacterial adaptation.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Desinfecção / Proteínas de Choque Térmico HSP70 / Proteínas de Escherichia coli / Escherichia coli K12 / Ondas Ultrassônicas Idioma: En Revista: Lett Appl Microbiol Assunto da revista: MICROBIOLOGIA Ano de publicação: 2017 Tipo de documento: Article País de afiliação: Malta

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Desinfecção / Proteínas de Choque Térmico HSP70 / Proteínas de Escherichia coli / Escherichia coli K12 / Ondas Ultrassônicas Idioma: En Revista: Lett Appl Microbiol Assunto da revista: MICROBIOLOGIA Ano de publicação: 2017 Tipo de documento: Article País de afiliação: Malta