Evaluation of a widely used culture-based method for detection of livestock-associated meticillin-resistant Staphylococcus aureus (MRSA), Denmark and Norway, 2014 to 2016.

Larsen, Jesper; Sunde, Marianne; Islam, Md Zohorul; Urdahl, Anne Margrete; Barstad, Aina Steihaug; Larsen, Anders Rhod; Grøntvedt, Carl Andreas; Angen, Øystein

Larsen, Jesper; Sunde, Marianne; Islam, Md Zohorul; Urdahl, Anne Margrete; Barstad, Aina Steihaug; Larsen, Anders Rhod; Grøntvedt, Carl Andreas; Angen, Øystein.

Afiliação

Larsen J; Statens Serum Institut, Copenhagen S, Denmark.
Sunde M; These authors contributed equally as first authors.
Islam MZ; These authors contributed equally as first authors.
Urdahl AM; The Norwegian Veterinary Institute, Oslo, Norway.
Barstad AS; Statens Serum Institut, Copenhagen S, Denmark.
Larsen AR; The Norwegian Veterinary Institute, Oslo, Norway.
Grøntvedt CA; The Norwegian Veterinary Institute, Oslo, Norway.
Angen Ø; Statens Serum Institut, Copenhagen S, Denmark.

Euro Surveill ; 22(28)2017 Jul 13.

Article em En | MEDLINE | ID: mdl-28749335

ABSTRACT

ABSTRACT

We evaluated a widely used culture-based method for detection of livestock-associated meticillin-resistant Staphylococcus aureus (LA-MRSA) in samples collected from pigs and the environment inside pig stables in Denmark and Norway. Selective enrichment in tryptic soy broth containing cefoxitin and aztreonam led to a high ratio of false-negative results (26%; 57/221). On this basis, we recommend reconsidering the use of selective enrichment for detection of LA-MRSA in animal and environmental samples.

Assuntos

Técnicas de Cultura/métodos; Staphylococcus aureus Resistente à Meticilina/isolamento & purificação; Infecções Estafilocócicas/diagnóstico; Doenças dos Suínos/microbiologia; Animais; Dinamarca; Gado; Noruega; Sensibilidade e Especificidade; Infecções Estafilocócicas/veterinária; Sus scrofa; Suínos

Palavras-chave

MRSA; detection; laboratory surveillance; livestock; meticillin-resistant Staphylococcus aureus; sensitivity

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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Infecções Estafilocócicas / Doenças dos Suínos / Técnicas de Cultura / Staphylococcus aureus Resistente à Meticilina Tipo de estudo: Diagnostic_studies / Evaluation_studies / Risk_factors_studies Limite: Animals País/Região como assunto: Europa Idioma: En Revista: Euro Surveill Assunto da revista: DOENCAS TRANSMISSIVEIS Ano de publicação: 2017 Tipo de documento: Article País de afiliação: Dinamarca

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