Regulation of Rab5 isoforms by transcriptional and post-transcriptional mechanisms in yeast.
FEBS Lett
; 591(18): 2803-2815, 2017 09.
Article
em En
| MEDLINE
| ID: mdl-28792590
ABSTRACT
Rab5 GTPases are master regulators of early endosome biogenesis and transport. The genome of Saccharomyces cerevisiae encodes three Rab5 proteins Vps21, the major isoform, Ypt52 and Ypt53. Here, we show that Vps21 is the most abundant Rab5 protein and Ypt53 is the least abundant. In stressed cells, Ypt53 levels increase but never exceed that of Vps21. Its induction requires the transcription factors Crz1 and Gis1. In growing cells, the expression of Ypt53 is suppressed by post-transcriptional mechanisms mediated by the untranslated regions of the YPT53 mRNA. Based on genetic experiments, these sequences appear to stimulate deadenylation, Pat1-activated decapping and Xrn1-mediated mRNA degradation. Once this regulation is bypassed, Ypt53 protein levels surpass Vps21, and Ypt53 is sufficient to maintain endosomal function and cell growth.
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Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Saccharomyces cerevisiae
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Proteínas rab5 de Ligação ao GTP
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Proteínas de Saccharomyces cerevisiae
Idioma:
En
Revista:
FEBS Lett
Ano de publicação:
2017
Tipo de documento:
Article
País de afiliação:
Áustria