Identification of a 35S U4/U6.U5 tri-small nuclear ribonucleoprotein (tri-snRNP) complex intermediate in spliceosome assembly.

Chen, Zhe; Gui, Bin; Zhang, Yu; Xie, Guojia; Li, Wanjin; Liu, Shumeng; Xu, Bosen; Wu, Chongyang; He, Lin; Yang, Jianguo; Yi, Xia; Yang, Xiaohan; Sun, Luyang; Liang, Jing; Shang, Yongfeng

Chen, Zhe; Gui, Bin; Zhang, Yu; Xie, Guojia; Li, Wanjin; Liu, Shumeng; Xu, Bosen; Wu, Chongyang; He, Lin; Yang, Jianguo; Yi, Xia; Yang, Xiaohan; Sun, Luyang; Liang, Jing; Shang, Yongfeng.

Afiliação

Chen Z; From the Key Laboratory of Carcinogenesis and Translational Research (Ministry of Education), Department of Biochemistry and Molecular Biology, School of Basic Medical Sciences, Peking University Health Science Center, Beijing 100191, China.
Gui B; From the Key Laboratory of Carcinogenesis and Translational Research (Ministry of Education), Department of Biochemistry and Molecular Biology, School of Basic Medical Sciences, Peking University Health Science Center, Beijing 100191, China.
Zhang Y; From the Key Laboratory of Carcinogenesis and Translational Research (Ministry of Education), Department of Biochemistry and Molecular Biology, School of Basic Medical Sciences, Peking University Health Science Center, Beijing 100191, China.
Xie G; From the Key Laboratory of Carcinogenesis and Translational Research (Ministry of Education), Department of Biochemistry and Molecular Biology, School of Basic Medical Sciences, Peking University Health Science Center, Beijing 100191, China.
Li W; From the Key Laboratory of Carcinogenesis and Translational Research (Ministry of Education), Department of Biochemistry and Molecular Biology, School of Basic Medical Sciences, Peking University Health Science Center, Beijing 100191, China.
Liu S; From the Key Laboratory of Carcinogenesis and Translational Research (Ministry of Education), Department of Biochemistry and Molecular Biology, School of Basic Medical Sciences, Peking University Health Science Center, Beijing 100191, China.
Xu B; From the Key Laboratory of Carcinogenesis and Translational Research (Ministry of Education), Department of Biochemistry and Molecular Biology, School of Basic Medical Sciences, Peking University Health Science Center, Beijing 100191, China.
Wu C; From the Key Laboratory of Carcinogenesis and Translational Research (Ministry of Education), Department of Biochemistry and Molecular Biology, School of Basic Medical Sciences, Peking University Health Science Center, Beijing 100191, China.
He L; From the Key Laboratory of Carcinogenesis and Translational Research (Ministry of Education), Department of Biochemistry and Molecular Biology, School of Basic Medical Sciences, Peking University Health Science Center, Beijing 100191, China.
Yang J; From the Key Laboratory of Carcinogenesis and Translational Research (Ministry of Education), Department of Biochemistry and Molecular Biology, School of Basic Medical Sciences, Peking University Health Science Center, Beijing 100191, China.
Yi X; From the Key Laboratory of Carcinogenesis and Translational Research (Ministry of Education), Department of Biochemistry and Molecular Biology, School of Basic Medical Sciences, Peking University Health Science Center, Beijing 100191, China.
Yang X; From the Key Laboratory of Carcinogenesis and Translational Research (Ministry of Education), Department of Biochemistry and Molecular Biology, School of Basic Medical Sciences, Peking University Health Science Center, Beijing 100191, China.
Sun L; From the Key Laboratory of Carcinogenesis and Translational Research (Ministry of Education), Department of Biochemistry and Molecular Biology, School of Basic Medical Sciences, Peking University Health Science Center, Beijing 100191, China.
Liang J; From the Key Laboratory of Carcinogenesis and Translational Research (Ministry of Education), Department of Biochemistry and Molecular Biology, School of Basic Medical Sciences, Peking University Health Science Center, Beijing 100191, China.
Shang Y; From the Key Laboratory of Carcinogenesis and Translational Research (Ministry of Education), Department of Biochemistry and Molecular Biology, School of Basic Medical Sciences, Peking University Health Science Center, Beijing 100191, China, yshang@hsc.pku.edu.cn.

J Biol Chem ; 292(44): 18113-18128, 2017 11 03.

Article em En | MEDLINE | ID: mdl-28878014

Assuntos

Processamento Alternativo; Antígenos de Neoplasias/metabolismo; Biogênese de Organelas; RNA Helicases/metabolismo; Proteínas de Ligação a RNA/metabolismo; Proteínas Repressoras/metabolismo; Spliceossomos/metabolismo; Proteases Específicas de Ubiquitina/metabolismo; Antígenos de Neoplasias/química; Antígenos de Neoplasias/genética; Corpos Enovelados/química; Corpos Enovelados/enzimologia; Corpos Enovelados/metabolismo; Células HeLa; Humanos; Imunoprecipitação; Células MCF-7; Microscopia Eletrônica de Transmissão; Microscopia de Fluorescência; Peso Molecular; Mutação; Coloração Negativa; Oligopeptídeos/genética; Oligopeptídeos/metabolismo; Multimerização Proteica; Estabilidade Proteica; RNA Helicases/química; RNA Helicases/genética; Proteínas de Ligação a RNA/química; Proteínas de Ligação a RNA/genética; Proteínas Recombinantes de Fusão/química; Proteínas Recombinantes de Fusão/metabolismo; Proteínas Repressoras/química; Proteínas Repressoras/genética; Ribonucleoproteína Nuclear Pequena U5/química; Ribonucleoproteína Nuclear Pequena U5/metabolismo; Spliceossomos/química; Spliceossomos/enzimologia; Proteases Específicas de Ubiquitina/química; Proteases Específicas de Ubiquitina/genética

Palavras-chave

Cajal body; RNA helicase; RNA splicing; alternative splicing; ribonuclear protein (RNP); snRNP assembly; spliceosome; tri-snRNP

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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Proteínas Repressoras / Biogênese de Organelas / Proteínas de Ligação a RNA / Spliceossomos / Processamento Alternativo / RNA Helicases / Proteases Específicas de Ubiquitina / Antígenos de Neoplasias Tipo de estudo: Diagnostic_studies / Prognostic_studies Idioma: En Revista: J Biol Chem Ano de publicação: 2017 Tipo de documento: Article País de afiliação: China

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