Expression and purification of an FGF9 fusion protein in E. coli, and the effects of the FGF9 subfamily on human hepatocellular carcinoma cell proliferation and migration.

Wang, Shen; Lin, Haipeng; Zhao, Tiantian; Huang, Sisi; Fernig, David G; Xu, Nuo; Wu, Fenfang; Zhou, Mi; Jiang, Chao; Tian, Haishan

Wang, Shen; Lin, Haipeng; Zhao, Tiantian; Huang, Sisi; Fernig, David G; Xu, Nuo; Wu, Fenfang; Zhou, Mi; Jiang, Chao; Tian, Haishan.

Afiliação

Wang S; School of Pharmaceutical Science, Wenzhou Medical University, Wenzhou, Zhejiang, 325035, China.
Lin H; School of Pharmaceutical Science, Wenzhou Medical University, Wenzhou, Zhejiang, 325035, China.
Zhao T; School of Pharmaceutical Science, Wenzhou Medical University, Wenzhou, Zhejiang, 325035, China.
Huang S; School of Pharmaceutical Science, Wenzhou Medical University, Wenzhou, Zhejiang, 325035, China.
Fernig DG; Department of Biochemistry, Institute of Integrative Biology, University of Liverpool, Liverpool, L69 7ZB, UK.
Xu N; Biomedicine Collaborative Innovation Center, Wenzhou University, Wenzhou, Zhejiang, 325035, China.
Wu F; Biomedicine Collaborative Innovation Center, Wenzhou University, Wenzhou, Zhejiang, 325035, China.
Zhou M; School of Pharmaceutical Science, Wenzhou Medical University, Wenzhou, Zhejiang, 325035, China.
Jiang C; School of Pharmaceutical Science, Wenzhou Medical University, Wenzhou, Zhejiang, 325035, China.
Tian H; School of Pharmaceutical Science, Wenzhou Medical University, Wenzhou, Zhejiang, 325035, China. Chaojiang10@hotmail.com.

Appl Microbiol Biotechnol ; 101(21): 7823-7835, 2017 Nov.

Article em En | MEDLINE | ID: mdl-28921304

Assuntos

Movimento Celular/efeitos dos fármacos; Proliferação de Células/efeitos dos fármacos; Fator 9 de Crescimento de Fibroblastos/metabolismo; Hepatócitos/efeitos dos fármacos; Proteínas Recombinantes de Fusão/metabolismo; Animais; Linhagem Celular; Escherichia coli/genética; Escherichia coli/metabolismo; Fator 9 de Crescimento de Fibroblastos/genética; Fator 9 de Crescimento de Fibroblastos/isolamento & purificação; Expressão Gênica; Humanos; Camundongos; Proteínas Recombinantes de Fusão/genética; Proteínas Recombinantes de Fusão/isolamento & purificação

Palavras-chave

Fusion expression; HuH7 cells; Mitogen activity; Recombinant human FGF9; Signal transduction mechanism; TEV protease cleavage

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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Proteínas Recombinantes de Fusão / Movimento Celular / Hepatócitos / Proliferação de Células / Fator 9 de Crescimento de Fibroblastos Limite: Animals / Humans Idioma: En Revista: Appl Microbiol Biotechnol Ano de publicação: 2017 Tipo de documento: Article País de afiliação: China

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