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Luciferases with Tunable Emission Wavelengths.
Hiblot, Julien; Yu, Qiuliyang; Sabbadini, Marina D B; Reymond, Luc; Xue, Lin; Schena, Alberto; Sallin, Olivier; Hill, Nicholas; Griss, Rudolf; Johnsson, Kai.
Afiliação
  • Hiblot J; Ecole Polytechnique Fédérale de Lausanne, EPFL, Institute of Chemical Sciences and Engineering, ISIC, NCCR in Chemical Biology, 1015, Lausanne, Switzerland.
  • Yu Q; Max-Planck-Institute for Medical Research, Department of Chemical Biology, 69120, Heidelberg, Germany.
  • Sabbadini MDB; Ecole Polytechnique Fédérale de Lausanne, EPFL, Institute of Chemical Sciences and Engineering, ISIC, NCCR in Chemical Biology, 1015, Lausanne, Switzerland.
  • Reymond L; Max-Planck-Institute for Medical Research, Department of Chemical Biology, 69120, Heidelberg, Germany.
  • Xue L; Ecole Polytechnique Fédérale de Lausanne, EPFL, Institute of Chemical Sciences and Engineering, ISIC, NCCR in Chemical Biology, 1015, Lausanne, Switzerland.
  • Schena A; Ecole Polytechnique Fédérale de Lausanne, EPFL, Institute of Chemical Sciences and Engineering, ISIC, NCCR in Chemical Biology, 1015, Lausanne, Switzerland.
  • Sallin O; Ecole Polytechnique Fédérale de Lausanne, EPFL, Institute of Chemical Sciences and Engineering, ISIC, NCCR in Chemical Biology, 1015, Lausanne, Switzerland.
  • Hill N; Max-Planck-Institute for Medical Research, Department of Chemical Biology, 69120, Heidelberg, Germany.
  • Griss R; Ecole Polytechnique Fédérale de Lausanne, EPFL, Institute of Chemical Sciences and Engineering, ISIC, NCCR in Chemical Biology, 1015, Lausanne, Switzerland.
  • Johnsson K; Ecole Polytechnique Fédérale de Lausanne, EPFL, Institute of Chemical Sciences and Engineering, ISIC, NCCR in Chemical Biology, 1015, Lausanne, Switzerland.
Angew Chem Int Ed Engl ; 56(46): 14556-14560, 2017 11 13.
Article em En | MEDLINE | ID: mdl-28941028
ABSTRACT
We introduce luciferases whose emission maxima can be tuned to different wavelengths by chemical labeling. The luciferases are chimeras of NanoLuc with either SNAP-tag or HaloTag7. Labeling of the self-labeling tag with a fluorophore shifts the emission maximum of NanoLuc to that of the fluorophore. Luciferases with tunable colors have applications as reporter genes, for the construction of biosensors and in bioimaging.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Luciferases Limite: Humans Idioma: En Revista: Angew Chem Int Ed Engl Ano de publicação: 2017 Tipo de documento: Article País de afiliação: Suíça
Texto completo
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Luciferases Limite: Humans Idioma: En Revista: Angew Chem Int Ed Engl Ano de publicação: 2017 Tipo de documento: Article País de afiliação: Suíça